School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621000, China.
State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang, 832000, China.
Biol Trace Elem Res. 2023 Apr;201(4):1717-1725. doi: 10.1007/s12011-022-03266-8. Epub 2022 May 4.
To investigate the response of different levels of molybdenum (Mo) fertilizer to Chinese Merino sheep (Junken Type) grazing on natural heavy metal-contaminated meadows, this study was carried out in the Bayanbulak Grassland lying in the northwest of Xinjiang Uygur Autonomous Region, China. A total of 24-hm polluted meadows were fenced and were randomly divided into four groups (3 replication/group and 2 hm/replication) applied 0-kg Mo, 1-kg Mo, 2-kg Mo, and 3-kg Mo (ammonium molybdate tetrahydrate) per hectare for the CON group, group I, group II, and group III, respectively. Seventy-two healthy 1-year-old Chinese Merino sheep (45.56 ± 2.35 kg) were randomly assigned to the tested pastures for 90 days. Compared with the CON group, the Mo content from fertilized groups and the Se content from group II and group III in serums and livers were significantly increased (P < 0.05), and the Cu content from fertilized groups in serums and livers was significantly decreased (P < 0.05). The levels of blood Hb and RBC, and the activities of serum SOD, CAT, GSH-Px, and Cp in group III, were significantly higher (P < 0.05) than those in the CON group, group I, and group II. Serum MDA content in group III was significantly lower (P < 0.05) than that in the other three groups. In summary, Mo fertilization improved the antioxidant capacity of grazing sheep and also reduced the toxic damage to Chinese Merino sheep grazing on natural grasslands contaminated by heavy metals, but Mo poisoning caused by excessive fertilization should be prevented.
为了研究不同水平的钼肥对在天然重金属污染草地上放牧的中国美利奴羊（军垦型）的响应，本研究在中国新疆维吾尔自治区西北部的巴音布鲁克草原进行。共围封了 24 公顷受污染的草地，随机分为 4 组（每组 3 个重复，每个重复 2 公顷），分别施入 0、1、2 和 3 公斤钼（四水合钼酸铵）/公顷，对照组（CON 组）、第 I 组、第 II 组和第 III 组。72 只健康的 1 岁中国美利奴羊（45.56±2.35 公斤）被随机分配到试验草地上放牧 90 天。与对照组相比，施肥组血清和肝脏中的 Mo 含量和第 II 组和第 III 组的 Se 含量显著增加（P<0.05），施肥组血清和肝脏中的 Cu 含量显著降低（P<0.05）。第 III 组血清 Hb 和 RBC 水平以及血清 SOD、CAT、GSH-Px 和 Cp 的活性显著高于对照组、第 I 组和第 II 组（P<0.05）。第 III 组血清 MDA 含量显著低于其他三组（P<0.05）。总之，钼肥提高了放牧绵羊的抗氧化能力，同时减轻了重金属污染天然草地放牧的中国美利奴羊的毒性损伤，但应防止过量施肥引起的钼中毒。
