School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
World Bank Poverty Alleviation Project Office in Guizhou, Southwest China, Guiyang, 550004, China.
Biol Trace Elem Res. 2021 Mar;199(3):989-995. doi: 10.1007/s12011-020-02214-8. Epub 2020 Jun 23.
To research responses of Chinese Merino Sheep (Junken type) to copper-deprived natural pasture, we analyzed mineral contents in soil, forage, and sheep tissues. Physiological and biochemical parameters were also determined. Results showed that copper concentrations in soil and forage from affected pastures were significantly lower than those in healthy ranges (P < 0.01). Copper contents in animal tissues (blood, liver, and wool) from affected Chinese Merino Sheep were also significantly lower than those in healthy sheep (P < 0.01). Hemoglobin levels, packed cell volume, mean corpuscular volume, and mean corpuscular hemoglobin from affected Chinese Merino Sheep were significantly lower than those in healthy animals (P < 0.01). Serum ceruloplasmin was significantly lower in affected Chinese Merino Sheep than that in healthy animals, while activities of lactate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase in serum were significantly higher in affected compared with healthy animals (P < 0.01). The levels of superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and catalase in serum were significantly lower, and the malondialdehyde content was significantly higher in affected compared with healthy sheep (P < 0.01). The levels of interleukin-2, interleukin 6, interleukin-1β, immunoglobulin A, immunoglobulin M, and immunoglobulin G in copper-deprived sheep were significantly lower than those in healthy animals (P < 0.01). Copper deprivation in forage not only influenced the mineral content in blood but also severely disrupted blood parameters (physiology, biochemistry, immunity, and antioxidant) in Chinese Merino Sheep.
为了研究中国美利奴羊（Junken 型）对缺铜天然牧场的反应，我们分析了土壤、饲料和羊组织中的矿物质含量。还测定了生理和生化参数。结果表明，受影响牧场土壤和饲料中的铜浓度明显低于健康范围（P<0.01）。受影响的中国美利奴羊组织（血液、肝脏和羊毛）中的铜含量也明显低于健康羊（P<0.01）。受影响的中国美利奴羊的血红蛋白水平、红细胞压积、平均红细胞体积和平均红细胞血红蛋白明显低于健康动物（P<0.01）。受影响的中国美利奴羊血清铜蓝蛋白明显低于健康动物，而血清中乳酸脱氢酶、碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶的活性明显高于健康动物（P<0.01）。血清中超氧化物歧化酶、谷胱甘肽过氧化物酶、总抗氧化能力和过氧化氢酶的水平明显降低，而丙二醛的含量明显升高（P<0.01）。受铜缺乏影响的绵羊白细胞介素-2、白细胞介素 6、白细胞介素 1β、免疫球蛋白 A、免疫球蛋白 M 和免疫球蛋白 G 的水平明显低于健康动物（P<0.01）。饲料中铜的缺乏不仅影响血液中的矿物质含量，而且严重破坏中国美利奴羊的血液参数（生理、生化、免疫和抗氧化）。
