从鼠胚胎干细胞生成唾液腺类器官。

Generation of Salivary Gland Organoids from Mouse Embryonic Stem Cells.

机构信息

Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Tokyo, Japan.

出版信息

Methods Mol Biol. 2022;2429:247-255. doi: 10.1007/978-1-0716-1979-7_16.

DOI:10.1007/978-1-0716-1979-7_16

Abstract

Salivary glands are exocrine glands composed of several cell types, including the ductal, acinar, and basal/myoepithelial cells. They play important roles in maintaining oral homeostasis and health. During early murine development, the salivary glands, which arise as epithelial buds, are produced from primitive oral epithelia through an interaction between the oral epithelium and mesenchyme.We recently reported that salivary gland organoids can be generated from mouse embryonic stem cells (ESCs). We recapitulated the process of embryonic salivary gland development using an organoid culture system. The mouse ESC-derived salivary gland organoids consisted of acinar-, ductal-, and myoepithelial-like cells. In this chapter, we describe a protocol for differentiating salivary gland organoids from ESCs .

摘要

唾液腺是由多种细胞类型组成的外分泌腺，包括导管、腺泡和基底/肌上皮细胞。它们在维持口腔内环境稳定和健康方面发挥着重要作用。在早期小鼠发育过程中，唾液腺是从原始口腔上皮通过口腔上皮和间充质之间的相互作用产生的上皮芽。我们最近报道，唾液腺类器官可以从小鼠胚胎干细胞（ESCs）中生成。我们使用类器官培养系统再现了胚胎唾液腺发育的过程。由小鼠 ESC 衍生的唾液腺类器官由腺泡、导管和肌上皮样细胞组成。在本章中，我们描述了从 ESCs 中分化唾液腺类器官的方案。