Lou Shangling, Guo Xiang, Liu Lian, Song Yan, Zhang Lei, Jiang Yuanzhong, Zhang Lushui, Sun Pengchuan, Liu Bao, Tong Shaofei, Chen Ningning, Liu Meng, Zhang Han, Liang Ruyun, Feng Xiaoqin, Zheng Yudan, Liu Huanhuan, Holdsworth Michael J, Liu Jianquan
Key Laboratory for Bio-resources and Eco-environment & State Key Lab of Hydraulics & Mountain River Engineering, College of Life Sciences, Sichuan University, Chengdu 610065, China.
School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UK.
Sci Adv. 2022 May 6;8(18):eabn8281. doi: 10.1126/sciadv.abn8281. Epub 2022 May 4.
Populations of widespread species are usually geographically distributed through contrasting stresses, but underlying genetic mechanisms controlling this adaptation remain largely unknown. Here, we show that in , allelic changes in the cis-regulatory elements, WT box and W box, in the promoter of a key transcription factor associated with oxygen sensing, RELATED TO AP 2.12 (RAP2.12), are responsible for differentially regulating tolerance to drought and flooding. These two cis-elements are regulated by different transcription factors that downstream of results in differential accumulation of hypoxia-responsive transcripts. The evolution from one cis-element haplotype to the other is associated with the colonization of humid environments from arid habitats. This gene thus promotes both drought and flooding adaptation via an adaptive mechanism that diversifies its regulation through noncoding alleles.
广泛分布物种的种群通常通过不同的压力在地理上进行分布，但控制这种适应性的潜在遗传机制在很大程度上仍不为人知。在这里，我们表明，在拟南芥中，与氧感应相关的关键转录因子RELATED TO AP 2.12（RAP2.12）启动子中的顺式调控元件WT盒和W盒的等位基因变化，负责差异调节对干旱和洪水的耐受性。这两个顺式元件由不同的转录因子调控，这些转录因子在缺氧诱导因子下游，导致缺氧反应转录本的差异积累。从一种顺式元件单倍型到另一种的进化与从干旱生境到潮湿环境的定殖有关。因此，该基因通过一种适应性机制促进了对干旱和洪水的适应，这种机制通过非编码等位基因使其调控多样化。
