Shienvold F L, Alejandro R, Latif Z, Mintz D H
Pancreas. 1986;1(5):385-96. doi: 10.1097/00006676-198609000-00001.
We have generated two panels of monoclonal antibodies (MoAbs) that represent a unique array of immunolabeling reagents specific for diverse cell surface and intracellular antigens of ductal epithelial cells (DEC) and of acinar cells (AC) within the human pancreas. Eight of the MoAbs are specific within the pancreas for the DEC plasma membrane (PM), three for DEC intracellular antigens, seven for AC-PM, and one for AC secretory granules. The MoAbs with PM reactivities have permitted us to substantially enhance our assessment of exocrine contamination in our human islet preparations. However, we have been unable to demonstrate consistently the effectiveness of these MoAbs in complement-mediated cytotoxicity protocols designed to improve the purity of our islet preparations by exocrine cytolysis. It is possible that primary cell isolates of pancreas or epithelial organs in general may lack inherent susceptibility to complement-mediated cytolysis or, instead, that this particular panel of MoAbs lacks the appropriate characteristics needed to achieve consistent complement-mediated cytolysis. We have also begun to explore the potentially broader applicability of the anti-DEC MoAbs as immunocytochemical tools and have observed intriguing patterns of cross-reactivities in other exocrine tissues, particularly bile duct epithelial cells in the liver and functionally discrete subsets of DEC in the eccrine sweat gland and the parotid gland. These homologous antigenic patterns, by virtue of their specificities for ductal tissue, most likely reflect the molecular bases for functions common to these tissues, e.g., active ion transport and mucin secretion. In addition, the anti-DEC and the anti-AC MoAbs exhibit patterns of binding specificity for human cell lines derived from pancreatic adenocarcinoma that suggest the potential value of these MoAbs in tumor diagnosis and therapy. We believe that these panels of MoAbs will find broad utility in immunopathology and in experimental approaches to questions regarding the development, normal function, and pathogenetic mechanisms in the human pancreas and other related organs as well.
我们制备了两组单克隆抗体（MoAbs），它们代表了一系列独特的免疫标记试剂，可特异性识别人类胰腺中导管上皮细胞（DEC）和腺泡细胞（AC）的多种细胞表面及细胞内抗原。其中8种MoAbs在胰腺内对DEC质膜（PM）具有特异性，3种针对DEC细胞内抗原，7种针对AC-PM，1种针对AC分泌颗粒。具有PM反应性的MoAbs使我们能够显著提高对人胰岛制备物中外分泌污染的评估。然而，在旨在通过外分泌细胞溶解提高胰岛制备物纯度的补体介导细胞毒性实验方案中，我们未能始终如一地证明这些MoAbs的有效性。胰腺或一般上皮器官的原代细胞分离物可能缺乏对补体介导细胞溶解的固有敏感性，或者相反，这组特定的MoAbs缺乏实现一致的补体介导细胞溶解所需的适当特性。我们还开始探索抗DEC MoAbs作为免疫细胞化学工具的潜在更广泛适用性，并在其他外分泌组织中观察到有趣的交叉反应模式，特别是肝脏中的胆管上皮细胞以及外分泌汗腺和腮腺中功能不同的DEC亚群。这些同源抗原模式因其对导管组织的特异性，很可能反映了这些组织共同功能的分子基础，例如主动离子转运和粘蛋白分泌。此外，抗DEC和抗AC MoAbs对源自胰腺腺癌的人细胞系表现出结合特异性模式，表明这些MoAbs在肿瘤诊断和治疗中的潜在价值。我们相信，这些MoAbs组将在免疫病理学以及关于人类胰腺和其他相关器官的发育、正常功能和致病机制问题的实验方法中具有广泛用途。
