Department of Periodontology, Rio de Janeiro State University, Brazil.
Department of Endodontic, Rio de Janeiro Federal University, Brazil.
Braz Dent J. 2022 Mar-Apr;33(2):68-72. doi: 10.1590/0103-6440202204591.
The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs.
世界卫生组织已宣布 SARS-CoV-2 的广泛传播及其相关疾病（COVID-19）为公共卫生紧急事件。检测病毒的标准金测试是 RT-PCR，从鼻咽拭子（NPS）样本中进行。然而，该测试可能会使患者感到不适，并且需要负责采集样本的卫生专业人员进行特定的培训和着装。因此，寻找替代方法来收集可能用于 COVID-19 诊断的样本是相关的。本研究旨在比较 NPS 和唾液样本的结果。从怀疑患有 COVID-19 的 189 名有症状的门诊患者中采集 NPS 和唾液样本，这些患者来到皮凯特·卡内罗综合诊所。使用 Bio-Gene DNA/RNA 病毒提取试剂盒（Bioclin®）进行 RNA 提取。使用实时逆转录-聚合酶链反应（RT-PCR）反应使用 Molecular SARS-CoV-2（E / RP）试剂盒（Bio-Manguinhos）。结果表明，142 个结果显示为不可检测（ND），而 47 个结果显示为可检测（D）。在 142 个“ND”中，137（94.4％）唾液样本获得了相同的结果，而 5 个样本（3.4％）为“D”。在 47 个“D”拭子样本中，35 个（74.4％）在唾液样本中显示出相同的结果。唾液测试的灵敏度为 0.74，特异性为 0.97。阳性预测值为 0.88，阴性预测值为 0.92。结果表明，与鼻咽拭子相比，使用唾液样本检测 Sars-CoV-2 具有较高的灵敏度和特异性。
