State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China.
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, Shandong, People's Republic of China; Engineering Research Center of Industrial Biocatalysis, Fujian Province Universities, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian, People's Republic of China; College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou 362000, People's Republic of China.
Int J Biol Macromol. 2022 Jun 15;210:622-629. doi: 10.1016/j.ijbiomac.2022.04.218. Epub 2022 May 1.
A molecular weight (Mw) controllable degradation strategy using the lyase WelR as the efficient tool was established, and the relationship between the Mw and the rheological properties and antioxidant activity of WL gum was systematically investigated. Four different WL samples WL1-WL4 with a gradient Mw change (from 4.70 × 10 to 1.45 × 10 Da) were obtained by controlling the enzymatic reaction conditions. As the Mw decreased, its apparent viscosity, intrinsic viscosity, viscous modulus (G″) and elastic modulus (G') decreased. More interestingly, in contrast to the native WL, the G″ of the degraded WL became higher than G'. Besides, the biodegraded WL samples possessed much higher hydroxyl radicals scavenging activity than the original WL. WL4 with the lowest Mw showed the highest HO radical scavenging activity, about 94.65% at 1 mg/mL. This work provided a useful method to obtain a series of WL samples with controllable Mw and properties, which will broaden the application of sphingans.
建立了一种利用裂合酶 WelR 进行分子量（Mw）可控降解的策略，系统研究了Mw 与 WL 胶流变性能和抗氧化活性之间的关系。通过控制酶反应条件，得到了 4 种不同的 WL 样品 WL1-WL4，其 Mw 呈梯度变化（从 4.70×10 到 1.45×10 Da）。随着 Mw 的降低，其表观黏度、特性黏度、黏性模量（G″）和弹性模量（G'）降低。更有趣的是，与天然 WL 相比，降解后的 WL 的 G″高于 G'。此外，生物降解的 WL 样品比原始 WL 具有更高的羟基自由基清除活性。Mw 最低的 WL4 表现出最高的 HO 自由基清除活性，在 1mg/mL 时约为 94.65%。这项工作提供了一种获得一系列具有可控 Mw 和性能的 WL 样品的有用方法，这将拓宽 sphingans 的应用范围。
