Department of Biology, Science Faculty, Ataturk University, 25240, Erzurum, Turkey.
Arch Microbiol. 2022 May 4;204(6):296. doi: 10.1007/s00203-022-02922-w.
The present study reports the production, extraction, partial characterization, biological activities and use in textile dyeing of an orange pigment from Metabacillus idriensis strain LipT27 (MN818522.2). Pigment production occurred with 400 µg carotenoid/g biomass yield. Characterization of the methanol extracts of pigment by UV-Visible spectrophotometry, TLC, NMR, and FTIR indicated that the pigment was a carotenoid group pigment. The pigment exhibited antibacterial activity against Yersinia enterocolitica, Staphylococcus aureus, and Escherichia coli and antioxidant potential in DPPH and ABTS assays. In addition, the crude pigment was used in dyeing cotton fabrics. The first carotenoid from M. idriensis, which has strong antibacterial and antioxidant properties and also has the potential to be used as a dyeing agent for textiles, suggests that it can be used as a natural colorant substitute to synthetic dyes.
本研究报告了一种橙色色素的生产、提取、部分特性鉴定、生物活性及其在纺织染色中的应用，该色素来自代谢芽孢杆菌LipT27 菌株（MN818522.2）。色素的产量为 400μg 类胡萝卜素/g 生物质。通过紫外-可见分光光度法、TLC、NMR 和 FTIR 对甲醇提取物中的色素进行了表征，表明该色素为类胡萝卜素组色素。该色素对肠炎耶尔森氏菌、金黄色葡萄球菌和大肠杆菌具有抗菌活性，并且在 DPPH 和 ABTS 测定中具有抗氧化潜力。此外，该粗色素还用于对棉织物进行染色。这是首次从 M. idriensis 中提取的类胡萝卜素，具有较强的抗菌和抗氧化性能，并且具有作为纺织品染色剂的潜力，表明它可以替代合成染料作为天然着色剂使用。
