State Key Laboratory of Crop Stress Biology for Arid Areas, College of Food Science and Engineering, Northwest A & F University, Yangling, 712100, Shaanxi, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A & F University, Yangling, 712100, Shaanxi, China.
BMC Plant Biol. 2022 May 4;22(1):228. doi: 10.1186/s12870-022-03557-1.
Anthocyanins, which account for color variation and remove reactive oxygen species, are widely synthesized in plant tissues and organs. Using targeted metabolomics and nanopore full-length transcriptomics, including differential gene expression analysis, we aimed to reveal potato leaf anthocyanin biosynthetic pathways in different colored potato varieties.
Metabolomics analysis revealed 17 anthocyanins. Their levels varied significantly between the different colored varieties, explaining the leaf color differences. The leaves of the Purple Rose2 (PurpleR2) variety contained more petunidin 3-O-glucoside and malvidin 3-O-glucoside than the leaves of other varieties, whereas leaves of Red Rose3 (RedR3) contained more pelargonidin 3-O-glucoside than the leaves of other varieties. In total, 114 genes with significantly different expression were identified in the leaves of the three potato varieties. These included structural anthocyanin synthesis-regulating genes such as F3H, CHS, CHI, DFR, and anthocyanidin synthase and transcription factors belonging to multiple families such as C3H, MYB, ERF, NAC, bHLH, and WRKY. We selected an MYB family transcription factor to construct overexpression tobacco plants; overexpression of this factor promoted anthocyanin accumulation, turning the leaves purple and increasing their malvidin 3-o-glucoside and petunidin 3-o-glucoside content.
This study elucidates the effects of anthocyanin-related metabolites on potato leaves and identifies anthocyanin metabolic network candidate genes.
花色变化和清除活性氧的花青苷广泛存在于植物组织和器官中。本研究采用靶向代谢组学和纳米孔全长转录组学，包括差异基因表达分析，旨在揭示不同颜色马铃薯品种叶片花青苷生物合成途径。
代谢组学分析共鉴定出 17 种花青苷，其在不同颜色品种间的含量差异显著，解释了叶片颜色的差异。Purple Rose2（PurpleR2）品种叶片中天竺葵素 3-O-葡萄糖苷和矢车菊素 3-O-葡萄糖苷的含量高于其他品种，而 Red Rose3（RedR3）品种叶片中的天竺葵素 3-O-葡萄糖苷含量高于其他品种。在这三个马铃薯品种的叶片中共鉴定出 114 个表达差异显著的基因，包括花色苷合成调控结构基因 F3H、CHS、CHI、DFR 和类黄酮 3-O-葡萄糖苷合成酶，以及属于多个家族的转录因子，如 C3H、MYB、ERF、NAC、bHLH 和 WRKY。我们选择一个 MYB 家族转录因子构建过表达烟草植株，过表达该因子促进了花色苷的积累，使叶片呈紫色，并增加了其矢车菊素 3-O-葡萄糖苷和天竺葵素 3-O-葡萄糖苷的含量。
本研究阐明了花色苷相关代谢物对马铃薯叶片的影响，鉴定了花色苷代谢网络候选基因。
