Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Nanyang Normal University, Nanyang, 473061, PR China.
Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Nanyang Normal University, Nanyang, 473061, PR China.
Microbes Infect. 2022 Sep;24(6-7):104983. doi: 10.1016/j.micinf.2022.104983. Epub 2022 May 2.
Since 2011, the Gyrovirus galga 1 (GyVg1, previously recognized as avian gyrovirus 2) strain has extensively been detected worldwide. The virus has been identified in several species, including chickens, humans, domestic cats, and snakes, especially in China. Therefore, in this study, the presence of GyVg1 was investigated in various zoo animals to determine whether it exists in various species in Nanyang, China. A total of 63 whole blood samples (1 sample from each animal) from 24 animal species were collected from the Nanyang Zoo. Eight different GyVg1 strains were identified in eight types of animals using polymerase chain reaction, and the full genome of each strain was sequenced. The whole genome of four GyVg1 strains, namely, HN2019-H1, HN2019-T1, HN2019-SD1, and HN2019-L1 identified in hippopotamus (Hippopotamus amphibius), tiger (Panthera tigris), sika deer (Cervus nippon), and lion (Panthera leo), respectively, comprised 2375 nucleotides (nt). The whole genome of the other strains, namely, HN2019-E1, HN2019-S1, HN2019-PF1, and HN2019-P1 identified in egret (Egretta garzetta), silver pheasant (Lophura nycthemera), peafowl (Pavonini), and common pheasants (Phasianus colchicus), respectively, comprised 2376 nt. Subsequently, a phylogenetic tree was constructed based on the 8 whole-genome sequence strains and 29 reference strains. These 37 strains were grouped into two major branches, group A and group B, and the 8 strains identified in this study were placed in group A. An analysis of the amino acids encoded by three open reading frames revealed some mutation sites unique to these eight strains. The substitution occurred at site 110 of viral protein 2 of HN2019-PF1, which is located in the highly conserved phosphatase motif WX7HX3CXCX5H (95-115aa). Recombination analysis revealed that, all these viral sequences were obtained as a result of recombination among the three GyVg1 strains (JL1511 and GS1512 from chickens and 17CC0810 from cat) from China and two strains (G17 from ferret of Hungary and RS-BR-15-2S from chicken of Brazil) from other countries. These findings indicate the complex evolution of GyVg1. Nevertheless, its transmission across the hosts is worth exploring.
自 2011 年以来，Gyrovirus galga 1（GyVg1，以前被认为是禽 Gyrovirus 2）株已在全球范围内广泛检测到。该病毒已在多种物种中被鉴定出来，包括鸡、人类、家猫和蛇，特别是在中国。因此，在这项研究中，研究人员调查了南阳动物园中的各种动物园动物中是否存在 GyVg1，以确定它是否存在于中国南阳的各种物种中。从南阳动物园的 24 个动物物种中采集了 63 份全血样本（每个动物 1 份）。使用聚合酶链反应从 8 种动物中鉴定出 8 种不同的 GyVg1 株，并对每种株的全长基因组进行了测序。在河马（Hippopotamus amphibius）、老虎（Panthera tigris）、梅花鹿（Cervus nippon）和狮子（Panthera leo）中分别鉴定出的 4 株 GyVg1 全长基因组 HN2019-H1、HN2019-T1、HN2019-SD1 和 HN2019-L1 由 2375 个核苷酸（nt）组成。在白鹭（Egretta garzetta）、银雉（Lophura nycthemera）、孔雀（Pavonini）和普通雉鸡（Phasianus colchicus）中分别鉴定出的其他 4 株 GyVg1 全长基因组 HN2019-E1、HN2019-S1、HN2019-PF1 和 HN2019-P1 由 2376 个 nt 组成。随后，根据 8 个全基因组序列株和 29 个参考株构建了系统发育树。这 37 个株分为两个主要分支，A 组和 B 组，本研究中鉴定的 8 个株被置于 A 组。对三个开放阅读框编码的氨基酸进行分析发现了这些 8 个株特有的一些突变位点。HN2019-PF1 的病毒蛋白 2 中的第 110 位发生了取代，该位置位于高度保守的磷酸酶基序 WX7HX3CXCX5H（95-115aa）中。重组分析表明，所有这些病毒序列都是中国的三株 GyVg1 株（鸡的 JL1511 和 GS1512 以及猫的 17CC0810）和两株（匈牙利的 G17 和巴西的鸡的 RS-BR-15-2S）重组的结果。这些发现表明 GyVg1 的进化复杂。然而，它在宿主之间的传播值得探索。
