Key Lab of High Performance Polymer Materials and Technology of MOE, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Institute of Advanced Materials, School of Chemistry and Chemical Engineering, and Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing 211189, China.
ACS Appl Mater Interfaces. 2022 May 18;14(19):22443-22453. doi: 10.1021/acsami.2c02851. Epub 2022 May 5.
Artificial supramolecular light-harvesting systems have expanded various properties on photoluminescence, enabling promising applications on cell imaging, especially for imaging in organelles. Supramolecular light-harvesting systems have been used for imaging in some organelles such as lysosome, Golgi apparatus, and mitochondrion, but developing a supramolecular light-harvesting platform for imaging two organelles synchronously still remains a great challenge. Here, we report a series of lower-rim dodecyl-modified sulfonato-calix[4]arene-mediated supramolecular light-harvesting platforms for efficient light-harvesting from three naphthalene diphenylvinylpyridiniums containing acceptors, Nile Red, and Nile Blue. All of the constructed supramolecular light-harvesting systems possess high light-harvesting efficiency. Furthermore, when the two acceptors are loaded simultaneously in a single light-harvesting donor system for imaging in human prostate cancer cells, organelle imaging in lysosome and Golgi apparatus can be realized at the same time with distinctive wavelength emission. Nile Red receives the light-harvesting energy from the donors, reaching orange emissions (625 nm) in lysosome while Nile Blue shows a near-infrared light-harvesting emission at 675 nm in Golgi apparatus in the same cells. Thus, the light harvesting system provides a pathway for synchronously efficient cell imaging in two distinct organelles with a single type of photoluminescent supramolecular nanoparticles.
人工超分子光捕获系统扩展了光致发光的各种性质，使其在细胞成像方面具有广阔的应用前景，特别是在细胞器成像方面。超分子光捕获系统已被用于成像一些细胞器，如溶酶体、高尔基体和线粒体，但开发用于同时对两个细胞器进行成像的超分子光捕获平台仍然是一个巨大的挑战。在这里，我们报告了一系列低边缘十二烷基修饰的磺基杯[4]芳烃介导的超分子光捕获平台，用于从三个含有萘二苯乙烯基吡啶盐受体、尼罗红和尼罗蓝的萘中高效收集光。所有构建的超分子光捕获系统都具有高光捕获效率。此外，当两个受体同时装载在单个光捕获供体系统中用于人前列腺癌细胞成像时，可以同时在溶酶体和高尔基体中实现细胞器成像，并且具有独特的波长发射。尼罗红从供体中接收光捕获能量，在溶酶体中发出橙色发射（625nm），而尼罗蓝在同一细胞的高尔基体中显示近红外光捕获发射，波长为 675nm。因此，该光收集系统为使用单一类型的光致发光超分子纳米粒子在两个不同的细胞器中进行同步高效细胞成像提供了一种途径。
