Ma Haitong, Wu Ming, Liu Hui, Wang Zhiwei, Guo Chenyan, Wang Shuangfei
College of Light Industry and Food Engineering, Guangxi University Nanning 530004 PR China
Key Laboratory of Clean Pulp and Paper and Pollution Control Nanning 530004 PR China.
RSC Adv. 2019 Jul 26;9(40):23086-23095. doi: 10.1039/c9ra03142a. eCollection 2019 Jul 23.
The effect of acetylene black (ACET) as additives on methane production, extracellular polymeric substances (EPS), microbial community structure and methanogenesis pathway during sludge anaerobic digestion (AD) was investigated in this study. The results indicated that the addition of 2 g L ACET resulted in a 44.36% increase in methane accumulation. ACET, which resulted in the increase of EPS and VSS/TSS by 4.71-50.64%, effectively improved the physicochemical properties of anaerobic granular sludge (AnGS). During anaerobic digestion, the high throughput sequencing presented direct evidence that the ACET increased microbial diversity and enriched functional microorganisms such as , , and , which can improve the hydrolysis acidification process and the acetotrophic pathway. These results were reaffirmed by applying metagenome inference and gene content inference (16S function prediction). Microscopically, significant enhancement in the AD efficiency can be due to the methanogenesis promoted by the ACET that can construct direct interspecies electron transfer (DIET) between the , , and . These results were expected to provide primary research data for improving the performance of anaerobic reactors and the development of microbial fuel cells.
本研究考察了乙炔黑（ACET）作为添加剂对污泥厌氧消化（AD）过程中甲烷产生、胞外聚合物（EPS）、微生物群落结构和产甲烷途径的影响。结果表明，添加2 g/L的ACET使甲烷累积量增加了44.36%。ACET使EPS和VSS/TSS增加了4.71 - 50.64%，有效改善了厌氧颗粒污泥（AnGS）的理化性质。在厌氧消化过程中，高通量测序提供了直接证据，表明ACET增加了微生物多样性，并富集了如 、 和 等功能微生物，这些微生物可以改善水解酸化过程和乙酸营养型途径。通过应用宏基因组推断和基因含量推断（16S功能预测）再次证实了这些结果。从微观角度看，AD效率的显著提高可能归因于ACET促进的产甲烷作用，其可以在 、 和 之间构建直接种间电子转移（DIET）。这些结果有望为提高厌氧反应器性能和微生物燃料电池的开发提供初步研究数据。
