Cai Shao-Lan, Yang Zi-Chuan, Wu Ke-Yang, Fan Cheng, Zhai Ling-Yan, Huang Nai-Han, Li Rong-Tian, Duan Wen-Jun, Chen Jin-Xiang
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University Guangzhou 510515 P. R. China
RSC Adv. 2019 May 17;9(27):15424-15430. doi: 10.1039/c9ra02028d. eCollection 2019 May 14.
We herein report an efficient Ag and S dual sensing scenario by a three-dimensional (3D) Cu-based metal-organic framework [Cu(Cdcbp)(bpea)] (MOF 1, HCdcbpBr = 3-carboxyl-(3,5-dicarboxybenzyl)-pyridinium bromide, bpea = 1,2-di(4-pyridinyl)ethane) shielded with a 5-carboxytetramethylrhodamine (TAMRA)-labeled C-rich single-stranded DNA (ss-probe DNA, P-DNA) as a fluorescent probe. The formed MOF-DNA probe, denoted as P-DNA@1, is able to sequentially detect Ag and S in one pot, with detection limits of 3.8 nM (for Ag) and 5.5 nM (for S), which are much more lower than the allowable Ag (0.5 μM) and S (0.6 μM) concentration in drinking water as regulated by World Health Organization (WHO). The detection method has been successfully applied to sense Ag and S in domestic, lake, and mineral water with satisfactory recoveries ranging from 98.2 to 107.3%. The detection mechanism was further confirmed by molecular simulation studies.
我们在此报告一种高效的银和硫双重传感方案，该方案采用一种三维（3D）铜基金属有机框架[Cu(Cdcbp)(bpea)]（MOF 1，HCdcbpBr = 3-羧基-(3,5-二羧基苄基)-吡啶溴化物，bpea = 1,2-二(4-吡啶基)乙烷），并用5-羧基四甲基罗丹明（TAMRA）标记的富含C的单链DNA（单链探针DNA，P-DNA）作为荧光探针进行屏蔽。所形成的MOF-DNA探针，记为P-DNA@1，能够在一锅反应中依次检测银和硫，检测限分别为3.8 nM（对于银）和5.5 nM（对于硫），这远低于世界卫生组织（WHO）规定的饮用水中银（0.5 μM）和硫（0.6 μM）的允许浓度。该检测方法已成功应用于检测生活用水、湖水和矿泉水中的银和硫，回收率令人满意，范围为98.2%至107.3%。分子模拟研究进一步证实了检测机制。
