Locatelli Alessandra, Iommarini Luisa, Graziadio Alessandra, Leoni Alberto, Porcelli Anna Maria, Iotti Stefano, Malucelli Emil, Francia Francesco, Venturoli Giovanni, Farruggia Giovanna
Dipartimento di Farmacia e Biotecnologie (FABIT), Università degli Studi di Bologna 40127 Bologna Italy
Centro Interdipartimentale di Ricerca Industriale (CIRI) Scienze della Vita e Tecnologie per la Salute, Università di Bologna 40100 Bologna Italy.
RSC Adv. 2019 May 16;9(27):15350-15356. doi: 10.1039/c9ra01800j. eCollection 2019 May 14.
A fluorescent derivative of trehalose with two dansyl groups (DAT) has been synthesized. It is characterised by a large Stokes shift, good permeability in human living cells and a well detectable fluorescent signal within the cells. Notably, in intestinal cells DAT is sequestered in vesicles induced by trehalose pre-treatment and colocalizes with lipid droplets.
已合成了一种带有两个丹磺酰基团的海藻糖荧光衍生物（DAT）。它具有大斯托克斯位移、在人类活细胞中良好的渗透性以及细胞内可良好检测到的荧光信号等特征。值得注意的是，在肠道细胞中，DAT被隔离在由海藻糖预处理诱导形成的囊泡中，并与脂滴共定位。
