Rosdahl V T, Jarløv J O, Knudsen A M
Acta Pathol Microbiol Immunol Scand B. 1986 Dec;94(6):423-7. doi: 10.1111/j.1699-0463.1986.tb03078.x.
A total of 8 penicillin-susceptible and 55 penicillin-resistant coagulase-negative Micrococcaceae were investigated for their beta-lactamase production by one quantitative and three qualitative methods. Seven of the 8 penicillin-susceptible strains had no beta-lactamase production; one had a very low production. Among the 55 penicillin-resistant strains, 54 produced beta-lactamase (mean 63 units/mg bacteria). Methicillin/gentamicin-resistant strains produced less enzyme than strains susceptible to these antibiotics. The percentage extracellularity (mean 18%) was lower than in Staphylococcus aureus, whereas the induction ratio (mean 56) was higher. No significant difference between the different species could be observed, but 70% of the strains were Staphylococcus epidermidis. Among the 55 beta-lactamase producing strains, the microbiological clover-leaf method detected 51, the iodometric method 40 and the chromogenic cephalosporin method 38. None of the three qualitative methods gave any false positive reactions.
采用一种定量方法和三种定性方法，对总共8株青霉素敏感和55株青霉素耐药的凝固酶阴性微球菌科细菌进行了β-内酰胺酶产生情况的研究。8株青霉素敏感菌株中，7株不产生β-内酰胺酶；1株产生量极低。在55株青霉素耐药菌株中，54株产生β-内酰胺酶（平均63单位/毫克细菌）。耐甲氧西林/庆大霉素的菌株产生的酶比这些抗生素敏感的菌株少。细胞外百分比（平均18%）低于金黄色葡萄球菌，而诱导率（平均56）较高。不同菌种之间未观察到显著差异，但70%的菌株为表皮葡萄球菌。在55株产生β-内酰胺酶的菌株中，微生物学三叶草叶法检测出51株，碘量法检测出40株，显色头孢菌素法检测出38株。这三种定性方法均未出现假阳性反应。
