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用于简单灵敏检测水中微囊藻毒素-LR的磷光免疫传感器。

Phosphorescent immunosensor for simple and sensitive detection of microcystin-LR in water.

作者信息

Qin Jin, Sun Xiaojie, Li Dongxia, Yan Guiqin

机构信息

Shanxi Normal University Linfen Shanxi 041000 China

出版信息

RSC Adv. 2019 Apr 25;9(22):12747-12754. doi: 10.1039/c9ra02141h. eCollection 2019 Apr 17.

DOI:10.1039/c9ra02141h
PMID:35515855
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9063644/
Abstract

A simple and sensitive Mn-ZnS quantum dot room-temperature phosphorescent immunosensor for detecting microcystin-LR was developed. This sensor adopted antigens and antibodies as recognition units and used Mn-ZnS RTP QDs as sensing materials to specifically bind with MC-LR. The structurally specific binding between the microcystin-LR antibody and MC-LR led to the aggregation of antibody-crosslinked QDs, and then the electrons of QDs would be transferred to the complex, leading to the phosphorescence quenching of QDs. The microcystin-LR antigen-antibody specific binding site was first analyzed. This phosphorescent immunosensor rapidly and sensitively detected microcystin-LR, with linear ranges of 0.2-1.5 μg L and 1.5-20 μg L and a detection limit of up to 0.024 μg L. Meanwhile, coexisting pollutants of microcystin-LR in water did not significantly interfere with microcystin-LR detection. The new sensor was applied to detect real water samples and showed high sensitivity and selectivity.

摘要

开发了一种用于检测微囊藻毒素-LR的简单灵敏的Mn-ZnS量子点室温磷光免疫传感器。该传感器采用抗原和抗体作为识别单元，并使用Mn-ZnS室温磷光量子点作为传感材料与微囊藻毒素-LR特异性结合。微囊藻毒素-LR抗体与微囊藻毒素-LR之间的结构特异性结合导致抗体交联量子点的聚集，然后量子点的电子会转移到复合物上，导致量子点的磷光猝灭。首次分析了微囊藻毒素-LR抗原-抗体特异性结合位点。这种磷光免疫传感器能快速灵敏地检测微囊藻毒素-LR，线性范围为0.2-1.5μg/L和1.5-20μg/L，检测限高达0.024μg/L。同时，水中微囊藻毒素-LR的共存污染物对微囊藻毒素-LR的检测没有显著干扰。该新型传感器应用于实际水样检测，具有高灵敏度和选择性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/a5cafec4e64f/c9ra02141h-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/73c0fbb72fc5/c9ra02141h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/3ad10b7462d9/c9ra02141h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/93afff7dd68d/c9ra02141h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/ccdeeda1f94c/c9ra02141h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/a5cafec4e64f/c9ra02141h-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/73c0fbb72fc5/c9ra02141h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/3ad10b7462d9/c9ra02141h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/93afff7dd68d/c9ra02141h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/ccdeeda1f94c/c9ra02141h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fc9/9063644/a5cafec4e64f/c9ra02141h-f5.jpg

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