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基于锰掺杂硫化锌量子点的“开启型”室温磷光生物传感器用于检测透明质酸

"Turn on" room-temperature phosphorescent biosensors for detection of hyaluronic acid based on manganese-doped ZnS quantum dots.

作者信息

Li Dongxia, Qin Jin, Lv Jinzhi, Yang Jiajia, Yan Guiqin

机构信息

Shanxi Normal University Linfen 041004 PR China

出版信息

RSC Adv. 2018 Jan 12;8(6):2873-2879. doi: 10.1039/c7ra11858a.

DOI:10.1039/c7ra11858a
PMID:35541178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9077376/
Abstract

Biosensors based on excellent optical properties of quantum dots (QDs) nanohybrids are efficient for biological detection. In this work, a room-temperature phosphorescent (RTP) PDAD-Mn-ZnS QDs biosensor was constructed with poly(diallyldimethylammonium chloride) (PDAD) as the modifier of MPA-capped Mn-ZnS QDs, and used to detect hyaluronic acid (HA). The newly-added HA induced severe electrostatic interaction with PDAD-Mn-ZnS QDs, leading to the aggregation between PDAD-Mn-ZnS QDs and HA and thereby enhancing RTP. The enhancement of RTP was proportional to the HA concentrations within certain ranges. On this basis, a high-performance HA sensor was built and this sensor had a detection limit of 0.03 μg mL and a detection range of 0.08-2.8 μg mL. This proposed RTP sensor can avoid interferences from the background fluorescence or scattering light of the matrix that are encountered in spectrofluorometry. Thus, this biosensor is potentially suitable for detection of HA in real samples without complicated pretreatment.

摘要

基于量子点（QDs）纳米杂化物优异光学性质的生物传感器在生物检测方面效率很高。在这项工作中，构建了一种室温磷光（RTP）的聚二烯丙基二甲基氯化铵（PDAD）-Mn-ZnS量子点生物传感器，其中聚二烯丙基二甲基氯化铵（PDAD）作为巯基丙酸封端的Mn-ZnS量子点的改性剂，并用于检测透明质酸（HA）。新添加的透明质酸与PDAD-Mn-ZnS量子点发生强烈的静电相互作用，导致PDAD-Mn-ZnS量子点与透明质酸之间发生聚集，从而增强室温磷光。在一定范围内，室温磷光的增强与透明质酸浓度成正比。在此基础上，构建了一种高性能的透明质酸传感器，该传感器的检测限为0.03 μg/mL，检测范围为0.08-2.8 μg/mL。所提出的室温磷光传感器可以避免荧光光谱法中遇到的基质背景荧光或散射光的干扰。因此，这种生物传感器可能适用于无需复杂预处理的实际样品中透明质酸的检测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/4c7e6259de94/c7ra11858a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/72f4e10ac88d/c7ra11858a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/0361d3c681bd/c7ra11858a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/8239967ad082/c7ra11858a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/33da9ca5dcd4/c7ra11858a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/4c7e6259de94/c7ra11858a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/72f4e10ac88d/c7ra11858a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/0361d3c681bd/c7ra11858a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/8239967ad082/c7ra11858a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/33da9ca5dcd4/c7ra11858a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0920/9077376/4c7e6259de94/c7ra11858a-f5.jpg

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