Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
Biosens Bioelectron. 2014 Nov 15;61:506-11. doi: 10.1016/j.bios.2014.06.004. Epub 2014 Jun 8.
Applying molecular imprinting techniques to the surface of functionalized quantum dots (QDs) allows the preparation of molecularly imprinted polymers (MIPs) with accessible, surface exposed binding sites and excellent optical properties. This paper demonstrates a new strategy for producing such hybrid organic-inorganic imprinted Mn-doped ZnS QDs for specific recognition of bovine hemoglobin. The technique provides surface grafting imprinting in aqueous solutions using amino modified Mn-doped ZnS QDs as supports, acrylamide and methacrylic acid as functional monomers, γ-methacryloxypropyl trimethoxy silane as the grafting agent, and bovine hemoglobin as a template. The amino propyl functional monomer layer directs the selective occurrence of imprinting polymerization at the QDs surface through copolymerization of grafting agents with functional monomers, but also acts as an assistive monomer to drive the template into the formed polymer shells to create effective recognition sites. Using MIP-QDs composites as a fluorescence sensing material, trace amounts of bovine hemoglobin are signaled with high selectivity by emission intensity changes of Mn-doped ZnS QDs, which is embedded into the imprinted polymers.
将分子印迹技术应用于功能化量子点（QDs）的表面，允许制备具有可及的、表面暴露的结合位点和优异光学性质的分子印迹聚合物（MIPs）。本文展示了一种生产这种用于特异性识别牛血红蛋白的混合有机-无机印迹 Mn 掺杂 ZnS QDs 的新策略。该技术在水溶液中使用氨基改性的 Mn 掺杂 ZnS QDs 作为载体、丙烯酰胺和甲基丙烯酸作为功能单体、γ-甲基丙烯酰氧基丙基三甲氧基硅烷作为接枝剂、牛血红蛋白作为模板进行表面接枝印迹。氨基丙基功能单体层通过接枝剂与功能单体的共聚，引导印迹聚合选择性地发生在 QDs 表面，同时还作为辅助单体将模板驱动到形成的聚合物壳中，以创建有效的识别位点。使用 MIP-QDs 复合材料作为荧光传感材料，通过嵌入印迹聚合物的 Mn 掺杂 ZnS QDs 的发射强度变化，对痕量牛血红蛋白进行高选择性信号检测。
