Luan Xingkun, Wang Haozheng, Xiang Zehong, Zhao Jiruo, Feng Ying, Shi Qiang, Gong Yumei, Wong Shing-Chung, Yin Jinghua
Shandong Provincial Key Laboratory of Olefin Catalysis and Polymerization, Key Laboratory of Rubber-Plastics (QUST), Ministry of Education/Shandong, Qindao University of Science and Technology Qingdao 266042 P. R. China
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 P. R. China
RSC Adv. 2019 Feb 11;9(10):5251-5258. doi: 10.1039/c8ra08215d.
Hemolysis of stored erythrocytes is a big obstacle for the development of new plasticizer-free polymer containers. Hemolysis is mainly caused by cell membrane oxidation and cation leaks from the intracellular fluid during storage. To construct an anti-hemolytic surface for a plasticizer-free polymer, we fabricated 2--α-d-glucopyranosyl-l-ascorbic acid (AA-2G)-loaded polycaprolactone (PCL)-crown ether micro/nanofibers on the surface of styrene--(ethylene--butylene)--styrene (SEBS). Our strategy is based on the sensitive response of the crown ether to leaked potassium, causing the release of AA-2G, the AA-2G can then remove the excess ROS, maintaining the Na/K-pump activity and the cell integrity. We demonstrated that the PCL-crown ether micro/nanofibers have been well prepared on the surface of SEBS; the micro/nanofibers provide a sensitive response to excess K and trigger the rapid release of AA-2G. AA-2G then acts as an antioxidant to reduce the excess ROS and maintain the Na/K-pump activity to mitigate cation leaks, resulting in the reduced hemolysis of the preserved erythrocytes. Our work thus provides a novel method for the development of plasticizer-free polymers for the storage of erythrocytes, and has the potential to be used to fabricate long-term anti-hemolytic biomaterials for use.
储存红细胞的溶血现象是新型无增塑剂聚合物容器开发中的一大障碍。溶血主要是由储存过程中细胞膜氧化和细胞内液阳离子泄漏引起的。为了构建无增塑剂聚合物的抗溶血表面，我们在苯乙烯-（乙烯-丁烯）-苯乙烯（SEBS）表面制备了负载2-α-D-吡喃葡萄糖基-L-抗坏血酸（AA-2G）的聚己内酯（PCL）-冠醚微/纳米纤维。我们的策略基于冠醚对泄漏钾的敏感响应，导致AA-2G释放，然后AA-2G可以去除过量的活性氧，维持钠钾泵活性和细胞完整性。我们证明了PCL-冠醚微/纳米纤维已在SEBS表面良好制备；微/纳米纤维对过量的钾有敏感响应并触发AA-2G的快速释放。然后AA-2G作为抗氧化剂减少过量的活性氧并维持钠钾泵活性以减轻阳离子泄漏，从而减少保存红细胞的溶血。因此，我们的工作为开发用于储存红细胞的无增塑剂聚合物提供了一种新方法，并且有潜力用于制造长期抗溶血生物材料以供使用。
