Shi Q, Hou J, Zhao C, Xin Z, Jin J, Li C, Wong S-C, Yin J
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
Department of Polymer, School of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, People's Republic of China.
Nanoscale. 2016 Jan 28;8(4):2022-9. doi: 10.1039/c5nr07070h.
A smart core-sheath nanofiber for non-adherent cell capture and release is demonstrated. The nanofibers are fabricated by single-spinneret electrospinning of poly(N-isopropylacrylamide) (PNIPAAm), polycaprolactone (PCL) and nattokinase (NK) solution blends. The self-assembly of PNIPAAm and PCL blends during the electrospinning generates the core-sheath PCL/PNIPAAm nanofibers with PNIPAAm as the sheath. The PNIPAAm-based core-sheath nanofibers are switchable between hydrophobicity and hydrophilicity with temperature change and enhance stability in the blood. When the nanofibers come in contact with blood, the NK is released from the nanofibers to resist platelet adhesion on the nanofiber surface, facilitating the direct capture and isolation of red blood cells (RBCs) from the blood above phase-transition temperature of PNIPAAm. Meanwhile, the captured RBCs are readily released from the nanofibers with temperature stimuli in an undamaged manner. The release efficiency of up to 100% is obtained while maintaining cellular integrity and function. This work presents promising nanofibers to effectively capture non-adherent cells and release for subsequent molecular analysis and diagnosis of single cells.
展示了一种用于非粘附细胞捕获和释放的智能核壳纳米纤维。这些纳米纤维通过对聚（N-异丙基丙烯酰胺）（PNIPAAm）、聚己内酯（PCL）和纳豆激酶（NK）溶液共混物进行单喷头静电纺丝制备而成。静电纺丝过程中PNIPAAm和PCL共混物的自组装产生了以PNIPAAm为鞘层的核壳PCL/PNIPAAm纳米纤维。基于PNIPAAm的核壳纳米纤维可随温度变化在疏水性和亲水性之间切换，并增强在血液中的稳定性。当纳米纤维与血液接触时，NK从纳米纤维中释放出来，以抵抗血小板在纳米纤维表面的粘附，便于在PNIPAAm的相变温度以上从血液中直接捕获和分离红细胞（RBC）。同时，捕获的RBC在温度刺激下以无损方式很容易从纳米纤维中释放出来。在保持细胞完整性和功能的同时，获得了高达100%的释放效率。这项工作展示了有前景的纳米纤维，可有效捕获非粘附细胞并释放，用于后续的单细胞分子分析和诊断。
