轻度氯化钠胁迫以时间依赖性方式影响葡萄球菌肠毒素C的转录并降低蛋白质表达。
Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression.
作者信息
Etter Danai, Ukowitz Christina, Eicher Corinne, Tasara Taurai, Johler Sophia
机构信息
Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
Laboratory of Food Microbiology, Institute for Food Nutrition and Health, ETH Zurich, Zurich, Switzerland.
出版信息
Front Microbiol. 2022 Apr 18;13:820067. doi: 10.3389/fmicb.2022.820067. eCollection 2022.
Enterotoxins (SEs) produced by are the cause of serious food intoxications. Staphylococcal enterotoxin C (SEC) is one of the main contributors, as it is often highly expressed. possesses a competitive growth advantage over accompanying bacterial flora under stress conditions encountered in foods, such as high NaCl concentrations. However, the influence of NaCl as an external stressor on SEC expression is still unclear. We investigated the influence of 4.5% NaCl on mRNA and SEC protein levels. A qRT-PCR assay revealed that NaCl stress leads to time-dependently decreased or elevated mRNA levels for most strains. SEC protein levels were generally decreased under NaCl stress. Our findings suggest that NaCl stress lowers overall SEC concentration and time-dependently affects mRNA levels.
由[未提及具体细菌名称]产生的肠毒素（SEs）是严重食物中毒的原因。葡萄球菌肠毒素C（SEC）是主要促成因素之一，因为它通常高度表达。在食品中遇到的应激条件下，如高NaCl浓度，[未提及具体细菌名称]比伴随的细菌菌群具有竞争生长优势。然而，NaCl作为外部应激源对SEC表达的影响仍不清楚。我们研究了4.5% NaCl对[未提及具体细菌名称]mRNA和SEC蛋白水平的影响。定量逆转录聚合酶链反应（qRT-PCR）分析表明，NaCl应激导致大多数菌株的mRNA水平随时间下降或升高。在NaCl应激下，SEC蛋白水平通常会下降。我们的研究结果表明，NaCl应激会降低总体SEC浓度，并随时间影响[未提及具体细菌名称]mRNA水平。
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