Gajewska Joanna, Zakrzewski Arkadiusz Józef, Chajęcka-Wierzchowska Wioleta, Zadernowska Anna
Department of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, 10-726 Olsztyn, Poland.
Pathogens. 2023 Jul 19;12(7):954. doi: 10.3390/pathogens12070954.
is one of the most important foodborne pathogens. has the capability to produce a variety of toxins, including staphylococcal enterotoxins (SEs). The aim of this study was to evaluate the survival rate of cells and analyze enterotoxins gene expression after exposure to osmotic stress and acidic/alkaline stress. To determine survival rates, the traditional plate counting method and flow cytometry were used. The expression levels of the enterotoxin genes were performed by quantitative reverse transcription PCR (RT-qPCR). Expression changes differed depending on the stressors chosen. The obtained results in this study showed the effect of critical food-related stress conditions on SE gene expression in . The study showed different expression levels of the tested enterotoxins genes depending on the stress. The most tested enterotoxin genes (, , and ) after exposure to pH = 4.5 stress have similar expression as in the optimal condition. After alkaline treatment (pH = 9.6), a similar expression gene value as for the optimal condition was observed. The analysis of gene expression in response to stress caused by NaCl, showed that the expression of decreased, whereas , , and genes increased. A significantly decreased expression of the gene was observed.
是最重要的食源性病原体之一。能够产生多种毒素,包括葡萄球菌肠毒素(SEs)。本研究的目的是评估细胞在暴露于渗透应激和酸性/碱性应激后的存活率,并分析肠毒素基因表达情况。为确定存活率,使用了传统平板计数法和流式细胞术。肠毒素基因的表达水平通过定量逆转录PCR(RT-qPCR)进行检测。表达变化因所选应激源而异。本研究获得的结果显示了与食品相关的关键应激条件对葡萄球菌肠毒素基因表达的影响。该研究表明,根据应激情况,所检测的肠毒素基因表达水平不同。暴露于pH = 4.5应激后,大多数检测的肠毒素基因(、和)的表达与最佳条件下相似。经碱性处理(pH = 9.6)后,观察到与最佳条件下相似的基因表达值。对NaCl引起的应激反应中的基因表达分析表明,基因的表达下降,而、和基因的表达增加。观察到基因的表达显著下降。