Liu Gang, Duan Zhiguang, Wang Pan, Fan Daidi, Zhu Chenhui
Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 China
Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 China.
RSC Adv. 2020 Jun 10;10(37):22234-22241. doi: 10.1039/d0ra03423a. eCollection 2020 Jun 8.
Fuzhuan brick tea (FBT) is a Chinese dark tea that is famous for its significant health benefits, in which () strains play a vital role in its postfermentation process. In this study, eurocristatine with hypoglycemic activity was discovered for the first time and purified from the spores of growing in FBT. Eurocristatine (98%) was obtained by D-101 macroporous resin-based column chromatography and preparative high performance liquid chromatography (HPLC) with a C column as the stationary phase and 35% acetonitrile in ultrapure water as the mobile phase. Hypoglycemic activity in a Hep-G2 cell hypoglycemic model was used as a screening indicator during purification. The chemical structure of eurocristatine was characterized by ESI/MS, H NMR and C NMR analyses. The antidiabetic effects of eurocristatine were verified in high-fat diet/streptozocin-induced type 2 diabetes mellitus (T2DM) rats. The results showed that eurocristatine significantly reduced fasting blood glucose. Our study demonstrated that eurocristatine, as a newly discovered hypoglycemic active substance, could be considered a potential candidate for the treatment of diabetes and its complications.
茯砖茶（FBT）是一种中国黑茶，因其显著的健康益处而闻名，其中（）菌株在其发酵后过程中起着至关重要的作用。在本研究中，首次从茯砖茶中生长的（）孢子中发现并纯化了具有降血糖活性的欧洲曲菌素。以D-101大孔树脂为柱色谱，以C柱为固定相，超纯水-35%乙腈为流动相，通过制备型高效液相色谱（HPLC）获得了98%的欧洲曲菌素。在纯化过程中，以Hep-G2细胞降血糖模型中的降血糖活性作为筛选指标。通过电喷雾电离质谱（ESI/MS）、氢核磁共振（1H NMR）和碳核磁共振（13C NMR）分析对欧洲曲菌素的化学结构进行了表征。在高脂饮食/链脲佐菌素诱导的2型糖尿病（T2DM）大鼠中验证了欧洲曲菌素的抗糖尿病作用。结果表明，欧洲曲菌素显著降低了空腹血糖。我们的研究表明，欧洲曲菌素作为一种新发现的降血糖活性物质，可被视为治疗糖尿病及其并发症的潜在候选药物。
