Bosca Federica, Corazzari Ingrid, Foglietta Federica, Canaparo Roberto, Durando Gianni, Pastero Linda, Arpicco Silvia, Dosio Franco, Zonari Daniele, Cravotto Giancarlo, Tagliapietra Silvia, Serpe Loredana, Turci Francesco, Barge Alessandro
Department of Drug Science and Technology, University of Turin Turin Italy
Department of Chemistry, University of Turin Turin Italy.
RSC Adv. 2020 Jun 8;10(37):21736-21744. doi: 10.1039/d0ra03944f.
Sonodynamic therapy (SDT) is an innovative anticancer approach, based on the excitation of a given molecule (usually a porphyrin) by inertial acoustic cavitation that leads to cell death the production of reactive oxygen species (ROS). This study aims to prepare and characterize nanosystems based on porphyrin grafted carbon nanotubes (SWCNTs), to understand some aspects of the mechanisms behind the SDT phenomenon. Three different porphyrins have been covalently linked to SWCNTs using either Diels-Alder or 1,3-dipolar cycloadditions. ROS production and cell viability have been evaluated upon ultrasound irradiation. Despite the low porphyrin content linked on the SWCNT, these systems have shown high ROS production and high tumour-cell-killing ability. The existence of a PET (photoinduced electron transfer)-like process would appear to be able to explain these observations. Moreover, the demonstrated ability to absorb light limits the impact of side effects due to light-excitation.
声动力疗法（SDT）是一种创新的抗癌方法，基于特定分子（通常是卟啉）通过惯性声空化作用被激发，进而导致细胞死亡并产生活性氧（ROS）。本研究旨在制备并表征基于卟啉接枝碳纳米管（SWCNTs）的纳米系统，以了解SDT现象背后机制的某些方面。使用狄尔斯-阿尔德反应或1,3-偶极环加成反应将三种不同的卟啉共价连接到SWCNTs上。在超声辐照下评估了ROS的产生和细胞活力。尽管连接在SWCNT上的卟啉含量较低，但这些系统已显示出高ROS产生和高肿瘤细胞杀伤能力。类似光诱导电子转移（PET）过程的存在似乎能够解释这些观察结果。此外，所证明的吸收光的能力限制了光激发引起的副作用的影响。
