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通过抑制脂肪酸合成从人多能干细胞中纯化心肌细胞和神经元。

Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of fatty acid synthesis.

机构信息

Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.

Department of Systems Medicine, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.

出版信息

STAR Protoc. 2022 Apr 28;3(2):101360. doi: 10.1016/j.xpro.2022.101360. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101360
PMID:35516845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9065422/
Abstract

Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol can be applied to hiPSCs and human embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Tohyama et al. (2016) and Tanosaki et al. (2020).

摘要

在这里，我们描述了一种通过代谢选择过程从人诱导多能干细胞（hiPSC）中获得高纯度心肌细胞和神经元的方案。与传统的纯化方案相比，这种方法更容易操作和扩展，并且更具成本效益。该方案可应用于 hiPSC 和人类胚胎干细胞。有关该方案使用和执行的完整详细信息，请参阅 Tohyama 等人（2016 年）和 Tanosaki 等人（2020 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/894587b3d800/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/40de8108fa35/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/354acc615f38/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/3920ccd604aa/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/8f64b27d41ab/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/894587b3d800/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/40de8108fa35/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/354acc615f38/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/3920ccd604aa/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/8f64b27d41ab/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d61b/9065422/894587b3d800/gr4.jpg

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