优化的功能性人诱导多能干细胞源性多巴胺能神经元生成方案。
Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons.
机构信息
CHU de Québec Research Center, Axe Neurosciences, Quebec City, QC, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, QC, Canada.
CHU de Québec Research Center, Axe Neurosciences, Quebec City, QC, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, QC, Canada.
出版信息
STAR Protoc. 2023 Sep 15;4(3):102486. doi: 10.1016/j.xpro.2023.102486. Epub 2023 Jul 28.
Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks. For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022)..
从人类诱导多能干细胞（hiPSCs）中生成功能性人类多巴胺能（DA）神经元是模拟与多巴胺相关的人类疾病和细胞替代疗法的关键工具。在此，我们介绍了一种将神经化转录因子（NGN2）编程和 DA 模式形成相结合的方案，用于将 hiPSCs 分化为成熟和功能齐全的诱导性 DA（iDA）神经元。我们描述了从 hiPSC 转导和神经诱导到 3 周内近乎纯、完全功能的 iDA 神经元的分化和成熟的步骤。有关此方案使用和执行的完整详细信息，请参阅 Sheta 等人（2022 年）。
Zotero 插件