研究真菌sp. CMU-LMA中Sch-642305的生物合成。

Trenti Francesco, Lebe Karen E, Adelin Emilie, Ouazzani Jamal, Schotte Carsten, Cox Russell J

Institute for Organic Chemistry and BMWZ, Leibniz Universität Hannover Schneiderberg 38 30167 Hannover Germany

Centre National de la Recherche Scientifique, Institut de Chimie des Substances Naturelles ICSN Gif-sur-Yvette France.

RSC Adv. 2020 Jul 21;10(46):27369-27376. doi: 10.1039/d0ra05311b.

Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-642305. Targeted gene disruptions together with reconstitution of the pathway in the heterologous host dissect key chemical steps and shed light on a series of oxidoreductions occuring in the pathway.

Sch-642305是一种由丝状真菌sp. CMU-LMA产生的不寻常的双环10元大环内酯，目前尚无其生物合成的证据。在此，我们生成了产生该物质的生物体的基因组草图序列，并发现了负责Sch-642305形成的生物合成基因簇。通过靶向基因破坏以及在异源宿主中重建该途径，剖析了关键的化学步骤，并揭示了该途径中发生的一系列氧化还原反应。