通过后基因组学方法发现真菌多烯大环内酯，揭示了真菌中转硫酯酶介导的聚酮化合物大环化反应。
The Discovery of Fungal Polyene Macrolides via a Postgenomic Approach Reveals a Polyketide Macrocyclization by trans-Acting Thioesterase in Fungi.
机构信息
Department of Life Sciences, Graduate School of Arts and Sciences , The University of Tokyo , 3-8-1 Komaba , Meguro-ku, Tokyo 153-8902 , Japan.
NMR Science and Development Division , RIKEN Spring-8 Center , 1-7-22 Suehiro-cho , Tsurumi-ku, Yokohama , Kanagawa 230-0045 , Japan.
出版信息
Org Lett. 2019 Jun 21;21(12):4788-4792. doi: 10.1021/acs.orglett.9b01674. Epub 2019 Jun 10.
Heterologous expression of a unique biosynthetic gene cluster (BGC) comprising a highly reducing polyketide synthase and stand-alone thioesterase genes in Aspergillus oryzae enabled us to isolate a novel 34-membered polyene macrolide, phaeospelide A (1). This is the first isolation of a fungal polyene macrolide and the first demonstration of fungal aliphatic macrolide biosynthetic machinery. In addition, sequence similarity network analysis demonstrated the existence of a large number of BGCs for novel fungal macrolides.
在米曲霉中异源表达一个独特的生物合成基因簇（BGC），其中包含一个高度还原的聚酮合酶和独立的硫酯酶基因，使我们能够分离到一种新型的 34 元聚烯大环内酯化合物，即黄皮孢菌素 A（1）。这是首次分离到真菌聚烯类大环内酯化合物，也是首次证明真菌脂肪族大环内酯类化合物的生物合成机制。此外，序列相似性网络分析表明存在大量新型真菌大环内酯化合物的 BGC。
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