Institute for Organic Chemistry, Leibniz University of Hannover, Schneiderberg 1B, 30167, Hannover, Germany.
BMWZ, Leibniz University of Hannover, Schneiderberg 38, 30167, Hannover, Germany.
Nat Commun. 2018 May 17;9(1):1963. doi: 10.1038/s41467-018-04364-9.
Xenovulene A is a complex fungal meroterpenoid, produced by the organism hitherto known as Acremonium strictum IMI 501407, for which limited biosynthetic evidence exists. Here, we generate a draft genome and show that the producing organism is previously unknown and should be renamed as Sarocladium schorii. A biosynthetic gene cluster is discovered which bears resemblance to those involved in the biosynthesis of fungal tropolones, with additional genes of unknown function. Heterologous reconstruction of the entire pathway in Aspergillus oryzae allows the chemical steps of biosynthesis to be dissected. The pathway shows very limited similarity to the biosynthesis of other fungal meroterpenoids. The pathway features: the initial formation of tropolone intermediates; the likely involvement of a hetero Diels-Alder enzyme; a terpene cyclase with no significant sequence homology to any known terpene cyclase and two enzymes catalysing oxidative-ring contractions.
黄孢原毛平革菌 A 是一种复杂的真菌生源二萜化合物，由目前已知的 Acremonium strictum IMI 501407 产生，该菌的生物合成证据有限。在这里，我们生成了一个草图基因组，并表明产生该化合物的生物体以前是未知的，应该被重新命名为 Sarocladium schorii。我们发现了一个生物合成基因簇，它与参与真菌色酮生物合成的基因簇具有相似性，还有一些具有未知功能的基因。在 Aspergillus oryzae 中异源重建整个途径，使得生物合成的化学步骤得以解析。该途径与其他真菌生源二萜化合物的生物合成非常相似。该途径的特点包括：最初形成色酮中间体；可能涉及杂 Diels-Alder 酶；萜烯环化酶与任何已知的萜烯环化酶没有显著的序列同源性，以及两种催化氧化环收缩的酶。
