Wang Er Hui, Yu Zeng Li, Bu Yong Jun, Xu Peng Wei, Xi Jin Yan, Liang Hai Yan
Department of Nutrition and Food Hygiene, College of Public Health, Xinxiang Medical University Xinxiang 453003 China
Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University Xinxiang 453003 China.
RSC Adv. 2019 Apr 16;9(21):11842-11850. doi: 10.1039/c9ra01017c. eCollection 2019 Apr 12.
The present study aimed to investigate the protective effects of grape seed proanthocyanidin extract (GSPE) on high-fat diet (HFD) induced testicular damage, oxidative stress, and apoptotic germ cell death. Male rats ( = 40) were randomly divided into four groups: the control group (treated with physiological saline), HFD group, HFD + GSPE (100 mg kg) group and HFD + GSPE (300 mg kg) group. Compared with the HFD group the rats of the GSPE-treated group showed improved serum testosterone levels, sperm quality and histological appearance of the testis tissue. Significant elevation of antioxidant enzyme (SOD, GSH, and GSH-Px) activities and remarkable reduction in MDA were also observed by GSPE administration, indicating that GSPE can decrease testicular oxidative stress. Finally, a significant reduction in spermatogenic cell apoptosis was detected by TUNEL assay. In summary, these results indicated that GSPE can suppress testicular dysfunction and this effect may be attributed to its antioxidant and anti-apoptotic properties. The current study indicates that GSPE can be considered a promising candidate for use as a drug or a food supplement to alleviate HFD-induced testicular dysfunction.
本研究旨在探讨葡萄籽原花青素提取物（GSPE）对高脂饮食（HFD）诱导的睾丸损伤、氧化应激和生精细胞凋亡死亡的保护作用。将40只雄性大鼠随机分为四组：对照组（用生理盐水处理）、HFD组、HFD + GSPE（100毫克/千克）组和HFD + GSPE（300毫克/千克）组。与HFD组相比，GSPE处理组的大鼠血清睾酮水平、精子质量和睾丸组织的组织学外观均有所改善。给予GSPE后还观察到抗氧化酶（超氧化物歧化酶、谷胱甘肽和谷胱甘肽过氧化物酶）活性显著升高，丙二醛显著降低，表明GSPE可降低睾丸氧化应激。最后，通过TUNEL检测发现生精细胞凋亡显著减少。综上所述，这些结果表明GSPE可抑制睾丸功能障碍，这种作用可能归因于其抗氧化和抗凋亡特性。当前研究表明，GSPE可被视为一种有前景的候选药物或食品补充剂，用于缓解HFD诱导的睾丸功能障碍。
