Li Shu Gang, Ding Yu Song, Niu Qiang, Xu Shang Zhi, Pang Li Juan, Ma Ru Lin, Jing Ming Xia, Feng Gang Ling, Liu Jia Ming, Guo Shu Xia
Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education), Shihezi University School of Medicine, Shihezi 832002, Xinjiang, China.
Biomed Environ Sci. 2015 Apr;28(4):272-80. doi: 10.3967/bes2015.038.
To determine the ability of grape seed proanthocyanidin extract (GSPE) in alleviating arsenic-induced reproductive toxicity.
Sixty male Kunming mice received the following treatments by gavage: normal saline solution (control); arsenic trioxide (ATO; 4 mg/kg); GSPE (400 mg/kg); ATO+GSPE (100 mg/kg); ATO+GSPE (200 mg/kg) and ATO+GSPE (400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), heme oxygenase 1 (HO1), glutathione S-transferase (GST), NAD(P)H dehydrogenase, and quinone 1 (NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase (SOD), glutathione (GSH), total antioxidative capability (T-AOC), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and reproductive indexes were analyzed.
ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO +GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of mRNA expression of Nrf2, HO, NQO1, and GST.
GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity.
确定葡萄籽原花青素提取物（GSPE）缓解砷诱导的生殖毒性的能力。
60只雄性昆明小鼠通过灌胃接受以下处理：生理盐水（对照组）；三氧化二砷（ATO；4mg/kg）；GSPE（400mg/kg）；ATO+GSPE（100mg/kg）；ATO+GSPE（200mg/kg）和ATO+GSPE（400mg/kg）。此后，处死小鼠并称重，检查睾丸的病理变化。通过实时PCR检测睾丸中核因子（红系衍生2）样2（Nrf2）、血红素加氧酶1（HO1）、谷胱甘肽S-转移酶（GST）、NAD（P）H脱氢酶和醌1（NQO1）的表达。分析超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、总抗氧化能力（T-AOC）、丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）和生殖指标。
与对照组相比，ATO处理的小鼠精子数量、睾丸体细胞指数以及SOD、GSH和T-AOC的活性水平显著降低。与ATO处理组相比，ATO+GSPE组的测量参数有所恢复。用ATO+高剂量GSPE处理的小鼠Nrf2、HO、NQO1和GST的mRNA表达水平最高。
GSPE通过激活Nrf2信号通路减轻小鼠睾丸中的氧化应激损伤，从而对抗砷诱导的生殖毒性。
