Niciński K, Witkowska E, Korsak D, Noworyta K, Trzcińska-Danielewicz J, Girstun A, Kamińska A
Institute of Physical Chemistry, Polish Academy of Sciences Kasprzaka 44/52 01-224 Warsaw Poland
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw Miecznikowa 1 02-096 Warsaw Poland.
RSC Adv. 2019 Jan 2;9(2):576-591. doi: 10.1039/c8ra08319c.
Surface-enhanced Raman scattering (SERS) has been intensively used recently as a highly sensitive, non-destructive, chemical specific, and label-free technique for a variety of studies. Here, we present a novel SERS substrate for: (i) the standard ultra-trace analysis, (ii) detection of whole microorganisms, and (iii) spectroelectrochemical measurements. The integration of electrochemistry and SERS spectroscopy is a powerful approach for investigation of the structural changes of adsorbed molecules, their redox properties, and for studying the intermediates of the reactions. We have developed a conductive SERS platform based on photovoltaic materials (PV) covered with a thin layer of silver, especially useful in electrochemical SERS analysis. These substrates named Ag/PV presented in this study combine crucial spectroscopic features such as high sensitivity, reproducibility, specificity, and chemical/physical stability. The designed substrates permit the label-free identification and differentiation of cancer cells (renal carcinoma) and pathogens ( and ). In addition, the developed SERS platform was adopted as the working electrode in an electrochemical SERS approach for -aminothiophenol (-ATP) studies. The capability to monitor in real-time the electrochemical changes spectro-electro-chemically has great potential for broadening the application of SERS.
表面增强拉曼散射（SERS）最近已被广泛用作一种高灵敏度、非破坏性、化学特异性且无需标记的技术，用于各种研究。在此，我们展示了一种新型SERS基底，用于：（i）标准超痕量分析，（ii）全微生物检测，以及（iii）光谱电化学测量。电化学与SERS光谱学的结合是研究吸附分子的结构变化、其氧化还原性质以及研究反应中间体的有力方法。我们基于覆盖有薄层银的光伏材料（PV）开发了一种导电SERS平台，在电化学SERS分析中特别有用。本研究中呈现的这些名为Ag/PV的基底结合了诸如高灵敏度、可重复性、特异性以及化学/物理稳定性等关键光谱特征。所设计的基底允许对癌细胞（肾癌）和病原体（以及）进行无标记识别和区分。此外，所开发的SERS平台被用作电化学SERS方法中用于对氨基硫酚（-ATP）研究的工作电极。以光谱电化学方式实时监测电化学变化的能力对于拓宽SERS的应用具有巨大潜力。
