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采用无标记定量蛋白质组学研究草莓果实经可控臭氧处理后的响应。

Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment.

作者信息

Chen Cunkun, Zhang Xiaojun, Zhang Huijie, Ban Zhaojun, Li Li, Dong Chenghu, Ji Haipeng, Xue Wentong

机构信息

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University Beijing China

College of Food Science and Nutritional Engineering, China Agricultural University Beijing China.

出版信息

RSC Adv. 2019 Jan 3;9(2):676-689. doi: 10.1039/c8ra08405j. eCollection 2019 Jan 2.

DOI:10.1039/c8ra08405j
PMID:35517630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9060875/
Abstract

To elucidate postharvest senescence in strawberry ( Duch. var. 'JingTaoXiang') fruit in response to ozone treatment at different concentrations (0, 2.144, 6.432, and 10.72 mg m), a label-free quantitative proteomic investigation was performed. Postharvest physiological quality traits including respiration rate, firmness, titratable acid, and anthocyanin content were characterized. The observed protein expression profile after storage was related to delayed senescence in strawberries. A total of 2413 proteins were identified in differentially treated strawberry fruits, and 382 proteins were differentially expressed between the four treatments on day 7 and the initial value (blank 0). Proteins related to carbohydrate and energy metabolism and anthocyanin biosynthesis, cell stress response, and fruit firmness were characterized and quantified. Ozone treatment at the concentration of 10.72 mg m effectively delayed the senescence of the strawberry. The proteomic profiles were linked to physiological traits of strawberry fruit senescence to provide new insights into possible molecular mechanisms.

摘要

为阐明不同浓度（0、2.144、6.432和10.72毫克/立方米）臭氧处理对草莓（品种‘京桃香’）采后衰老的影响，进行了一项无标记定量蛋白质组学研究。对包括呼吸速率、硬度、可滴定酸和花青素含量在内的采后生理品质性状进行了表征。储存后观察到的蛋白质表达谱与草莓衰老延迟有关。在不同处理的草莓果实中总共鉴定出2413种蛋白质，在第7天的四种处理与初始值（空白0）之间有382种蛋白质差异表达。对与碳水化合物和能量代谢、花青素生物合成、细胞应激反应和果实硬度相关的蛋白质进行了表征和定量。10.72毫克/立方米浓度的臭氧处理有效地延缓了草莓的衰老。蛋白质组学图谱与草莓果实衰老的生理特性相关联，为可能的分子机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/431e9e468353/c8ra08405j-f12.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/22e5f1861fbb/c8ra08405j-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/d4c76ade78fc/c8ra08405j-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/0aef5570e68e/c8ra08405j-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/16f84c06f1c2/c8ra08405j-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/9dfb2ccb9af6/c8ra08405j-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/81ace410cb07/c8ra08405j-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/431e9e468353/c8ra08405j-f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/4b009f8d5385/c8ra08405j-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/be02addacf92/c8ra08405j-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/b76cf7a9115f/c8ra08405j-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/af784c891a1d/c8ra08405j-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/1e6fe5b74dfc/c8ra08405j-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/22e5f1861fbb/c8ra08405j-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/d4c76ade78fc/c8ra08405j-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/0aef5570e68e/c8ra08405j-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/16f84c06f1c2/c8ra08405j-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/9dfb2ccb9af6/c8ra08405j-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/81ace410cb07/c8ra08405j-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ac1/9060875/431e9e468353/c8ra08405j-f12.jpg

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2
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3
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4
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