Yokoyama Ryusuke, Kuki Hiroaki, Kuroha Takeshi, Nishitani Kazuhiko
Laboratory of Plant Cell Wall Biology, Graduate School of Life Sciences, Tohoku University, Sendai 890-8578, Japan.
Proteomes. 2016 Nov 17;4(4):34. doi: 10.3390/proteomes4040034.
The development of a range of sub-proteomic approaches to the plant cell wall has identified many of the cell wall proteins. However, it remains difficult to elucidate the precise biological role of each protein and the cell wall dynamics driven by their actions. The plant protoplast provides an excellent means not only for characterizing cell wall proteins, but also for visualizing the dynamics of cell wall regeneration, during which cell wall proteins are secreted. It therefore offers a unique opportunity to investigate the de novo construction process of the cell wall. This review deals with sub-proteomic approaches to the plant cell wall through the use of protoplasts, a methodology that will provide the basis for further exploration of cell wall proteins and cell wall dynamics.
一系列针对植物细胞壁的亚蛋白质组学方法的发展已经鉴定出了许多细胞壁蛋白。然而，要阐明每种蛋白质的确切生物学作用以及由其作用驱动的细胞壁动态变化仍然很困难。植物原生质体不仅为表征细胞壁蛋白提供了一种极好的手段，而且还为观察细胞壁再生过程中的动态变化提供了可能，在此过程中细胞壁蛋白会被分泌出来。因此，它为研究细胞壁的从头构建过程提供了一个独特的机会。本综述通过使用原生质体来探讨针对植物细胞壁的亚蛋白质组学方法，这种方法将为进一步探索细胞壁蛋白和细胞壁动态变化提供基础。
