Fukushi Mayu, Kinoshita Keita, Yamada Masumi, Yajima Yuya, Utoh Rie, Seki Minoru
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University 1-33 Yayoi-cho, Inage-ku 263-8522 Japan
RSC Adv. 2019 Mar 19;9(16):9136-9144. doi: 10.1039/c9ra00257j. eCollection 2019 Mar 15.
Vascular tissue models created are of great utility in the biomedical research field, but versatile, facile strategies are still under development. In this study, we proposed a new approach to prepare vascular tissue models in PDMS-based composite channel structures embedded with barium salt powders. When a cell-containing hydrogel precursor solution was continuously pumped in the channel, the precursor solution in the vicinity of the channel wall was selectively gelled because of the barium ions as the gelation agent supplied to the flow. Based on this concept, we were able to prepare vascular tissue models, with diameters of 1-2 mm and with tunable morphologies, composed of smooth muscle cells in the hydrogel matrix and endothelial cells on the lumen. Perfusion culture was successfully performed under a pressurized condition of ∼120 mmHg. The presented platform is potentially useful for creating vascular tissue models that reproduce the physical and morphological characteristics similar to those of vascular tissues .
所创建的血管组织模型在生物医学研究领域具有很大的实用性，但通用、简便的策略仍在开发中。在本研究中，我们提出了一种新方法，用于在嵌入钡盐粉末的基于聚二甲基硅氧烷（PDMS）的复合通道结构中制备血管组织模型。当将含细胞的水凝胶前体溶液连续泵入通道时，由于作为凝胶剂的钡离子供应到流动中，通道壁附近的前体溶液会选择性地凝胶化。基于这一概念，我们能够制备直径为1 - 2毫米且形态可调的血管组织模型，该模型由水凝胶基质中的平滑肌细胞和管腔内的内皮细胞组成。在约120 mmHg的加压条件下成功进行了灌注培养。所展示的平台对于创建能够再现与血管组织相似的物理和形态特征的血管组织模型可能具有实用性。
