Baghbanzadeh Amir, Baghbani Elham, Hajiasgharzadeh Khalil, Noorolyai Saeed, Khaze Vahid, Mansoori Behzad, Shirmohamadi Masoud, Baradaran Behzad, Mokhtarzadeh Ahad
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2022 Jan;12(1):169-175. doi: 10.34172/apb.2022.018. Epub 2020 Oct 19.
is one of the well-known tumor suppressor miRNAs in the body but in many cases, its expression became reduced in patients suffering from gastric cancer (GC). The main purpose of this study was to restore the function of this miRNA in human GC cells and investigating the effects of enhanced expression of on proliferation, apoptosis, and migration of GC cells upon in vitro transfection. The KATO III GC cells were treated with 100 nM of or negative control sequences. Following that, the MTT assay, flow cytometry assay, and wound-healing assay were applied to estimate the impacts of enhanced expression of this miRNA on the viability, apoptosis, and migration rate of the cells, respectively. Moreover, the total RNA was isolated and alterations in the mRNA expression ratio of migratory genes were measured by qRT-PCR techniques. The findings designated that enhanced expression of suppressed the migratory ability of the cells, but had no significant effects on cell survival or apoptosis of the transfected cells. In addition, this inhibitory function of on the migration rate of the KATO III cell line occurs with concurrent suppression of vimentin and MMP-9 gene expression. It can be concluded that negatively influences the migratory ability of the cancerous cells and restoring its effects can be regarded as a promising target of future therapeutic interventions, especially for GC metastasis.
是体内著名的肿瘤抑制性微小RNA之一，但在许多情况下，其表达在胃癌（GC）患者中会降低。本研究的主要目的是在人GC细胞中恢复这种微小RNA的功能，并研究体外转染后其表达增强对GC细胞增殖、凋亡和迁移的影响。用100 nM的该微小RNA或阴性对照序列处理KATO III GC细胞。随后，分别应用MTT法、流式细胞术和伤口愈合试验来评估这种微小RNA表达增强对细胞活力、凋亡和迁移率的影响。此外，提取总RNA，并通过qRT-PCR技术测量迁移基因mRNA表达比率的变化。研究结果表明，该微小RNA表达增强抑制了细胞的迁移能力，但对转染细胞的存活或凋亡没有显著影响。此外，该微小RNA对KATO III细胞系迁移率的这种抑制作用伴随着波形蛋白和MMP-9基因表达的同时抑制。可以得出结论，该微小RNA对癌细胞的迁移能力有负面影响，恢复其作用可被视为未来治疗干预的一个有前景的靶点，尤其是对于GC转移。
