Hernandez Yulan, González-Pastor Rebeca, Belmar-Lopez Carolina, Mendoza Gracia, de la Fuente Jesus M, Martin-Duque Pilar
Instituto de Nanociencia de Aragón (INA), Universidad de Zaragoza 50018 Spain.
Instituto Aragonés de Ciencias de la Salud 50009 Zaragoza Spain
RSC Adv. 2019 Jan 10;9(3):1327-1334. doi: 10.1039/c8ra09088b. eCollection 2019 Jan 9.
Mesenchymal stem cells (MSCs) are adult pluripotent cells with the plasticity to be converted into different cell types. Their self-renewal capacity, relative ease of isolation, expansion and inherent migration to tumors, make them perfect candidates for cell therapy against cancer. However, MSCs are notoriously refractory to adenoviral infection, mainly because CAR (Coxsackie-Adenovirus Receptor) expression is absent or downregulated. Over the last years, nanoparticles have attracted a great deal of attention as potential vehicle candidates for gene delivery, but with limited effects on their own. Our data showed that the use of positively charged 14 nm gold nanoparticles either functionalized with arginine-glycine-aspartate (RGD) motif or not, increases the efficiency of adenovirus infection in comparison to commercial reagents without altering cell viability or cell phenotype. This system represents a simple, efficient and safe method for the transduction of MSCs, being attractive for cancer gene and cell therapies.
间充质干细胞（MSCs）是具有可塑性的成年多能细胞，能够转化为不同的细胞类型。它们的自我更新能力、相对容易分离、扩增以及向肿瘤的固有迁移特性，使其成为癌症细胞治疗的理想候选者。然而，众所周知，MSCs对腺病毒感染具有抗性，主要原因是缺乏或下调了柯萨奇病毒-腺病毒受体（CAR）的表达。在过去几年中，纳米颗粒作为潜在的基因递送载体受到了广泛关注，但单独使用时效果有限。我们的数据表明，与市售试剂相比，使用带正电荷的14纳米金纳米颗粒（无论是否用精氨酸-甘氨酸-天冬氨酸（RGD）基序功能化）可提高腺病毒感染效率，同时不会改变细胞活力或细胞表型。该系统代表了一种简单、高效且安全的MSCs转导方法，对癌症基因治疗和细胞治疗具有吸引力。
