Chair of Biochemistry and Molecular Medicine, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University , Witten, Germany .
Hum Gene Ther. 2017 Oct;28(10):820-832. doi: 10.1089/hum.2017.139.
Adenovirus-based vectors comprise the most frequently used vector type in clinical studies to date. Both intense lab research and insights from the clinical trials reveal the importance of a comprehensive understanding of vector-host interactions. Especially for systemic intravenous adenovirus vector delivery, it is paramount to develop safe and efficacious vectors. Very early vector-host interactions that take place in blood long before the first cell is being transduced are phenomena triggered by the surface, shape, and size of the adenovirus vector particles. Not surprisingly, a multitude of different technologies ranging from genetics to chemistry has been developed to alter the adenovirus vector surface. In this review, we discuss the most important technologies and evaluate them for their suitability to overcome hurdles imposed by early vector-host interactions.
腺病毒载体是迄今为止在临床研究中使用最频繁的载体类型。深入的实验室研究和临床试验的结果都揭示了全面了解载体-宿主相互作用的重要性。特别是对于全身静脉注射腺病毒载体递送，开发安全有效的载体至关重要。非常早期的载体-宿主相互作用发生在血液中，即在第一个细胞被转导之前，这些现象是由腺病毒载体颗粒的表面、形状和大小引发的。毫不奇怪，已经开发出了从遗传学到化学的多种不同技术来改变腺病毒载体的表面。在这篇综述中，我们讨论了最重要的技术，并评估了它们克服早期载体-宿主相互作用所带来的障碍的适用性。
