Ren Yuebing, Song Haibo, Wu Yuanpeng, Ma Xiaochun, Yu Xuezhong, Liu Jia, Sun Jianhui, Zhang Zhicheng
Department of Cardiology, Dongying People's Hospital Dongying Shandong 257091 China
RSC Adv. 2020 Aug 13;10(50):29780-29788. doi: 10.1039/d0ra04811a. eCollection 2020 Aug 10.
Anticancer oligopeptides are rarely studied because they are often present in very low concentrations in a complex matrix. In the current study, twelve oligopeptides were isolated and the amino acid sequence identified from the centipede. MTT results indicated that Trp-Gly-His-Glu (CO-10) showed excellent anti-proliferative potency against chondrosarcoma cells . Further study showed that CO-10 induced SW1353 cells apoptosis and blocked cell cycle in the G0/G1 phase. Further, results demonstrate that the apoptotic and cytotoxic effects of CO-10 are mediated by the intrinsic mitochondria-mediated apoptotic pathway, which in turn causes the release of cytochrome c and the activation of caspases. This study will be important for the development of pharmaceutical anticancer peptides from natural products as anticancer agents against chondrosarcoma.
抗癌寡肽很少被研究，因为它们在复杂基质中的浓度通常很低。在本研究中，从蜈蚣中分离出12种寡肽并鉴定了其氨基酸序列。MTT结果表明，Trp-Gly-His-Glu（CO-10）对软骨肉瘤细胞具有优异的抗增殖能力。进一步研究表明，CO-10诱导SW1353细胞凋亡并将细胞周期阻滞在G0/G1期。此外，结果表明CO-10的凋亡和细胞毒性作用是由内在的线粒体介导的凋亡途径介导的，这反过来又导致细胞色素c的释放和半胱天冬酶的激活。这项研究对于从天然产物开发用于对抗软骨肉瘤的药用抗癌肽作为抗癌剂具有重要意义。
