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用于合成羟基雌马酚的单加氧酶催化区域选择性羟基化反应。

Monooxygenase-catalyzed regioselective hydroxylation for the synthesis of hydroxyequols.

作者信息

Hashimoto Takafumi, Nozawa Daiki, Mukai Katsuyuki, Matsuyama Akinobu, Kuramochi Kouji, Furuya Toshiki

机构信息

Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science 2641 Yamazaki Noda Chiba 278-8510 Japan

Daicel Corporation 2-18-1 Konan, Minato-ku Tokyo 108-8230 Japan.

出版信息

RSC Adv. 2019 Jul 15;9(38):21826-21830. doi: 10.1039/c9ra03913a. eCollection 2019 Jul 11.

DOI:10.1039/c9ra03913a
PMID:35518870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9066559/
Abstract

Monooxygenases exhibiting high activity and differing regioselectivity for the dietary isoflavone metabolite equol were discovered among enzymes in the HpaBC family by a genome mining approach. These enzymes enabled the one-step product-selective synthesis of 3'- and 6-hydroxyequols from equol and molecular oxygen.

摘要

通过基因组挖掘方法，在HpaBC家族的酶中发现了对膳食异黄酮代谢产物雌马酚具有高活性且区域选择性不同的单加氧酶。这些酶能够从雌马酚和分子氧一步法进行产物选择性合成3'-羟基雌马酚和6-羟基雌马酚。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/27ffce00add2/c9ra03913a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/fbfc67d26be0/c9ra03913a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/e981cc6b953d/c9ra03913a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/07485d8e5ad5/c9ra03913a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/b7ab90b260a1/c9ra03913a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/27ffce00add2/c9ra03913a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/fbfc67d26be0/c9ra03913a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/e981cc6b953d/c9ra03913a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/07485d8e5ad5/c9ra03913a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/b7ab90b260a1/c9ra03913a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e7a/9066559/27ffce00add2/c9ra03913a-f4.jpg

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