酵母M1双链RNA中编码的前毒素基因的分析与利用

Analysis and utilization of the preprotoxin gene encoded in the M1 double-stranded RNA of yeast.

作者信息

Skipper N

出版信息

Basic Life Sci. 1986;40:215-26. doi: 10.1007/978-1-4684-5251-8_17.

PMID:3551915

Abstract

The yeast type 1 toxin is a secreted protein which, along with immunity to the toxin, is encoded by an encapsidated double-stranded RNA (dsRNA) as a precursor protein, the preprotoxin. This chapter reviews recent work that has led to sequencing of complementary DNA (cDNA) copies of the preprotoxin gene, expression of toxin and toxin-immunity from cDNA, and use of the cDNA to effect secretion from yeast of a bacterial cellulase.

摘要

酵母1型毒素是一种分泌蛋白，它与对该毒素的免疫性一起，由一种衣壳化双链RNA（dsRNA）作为前体蛋白（即前原毒素）进行编码。本章综述了近期的研究工作，这些工作导致了前原毒素基因互补DNA（cDNA）拷贝的测序、从cDNA表达毒素和毒素免疫性，以及利用该cDNA实现细菌纤维素酶在酵母中的分泌。