酵母M1双链RNA前原毒素编码区的克隆与测序
Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA.
作者信息
Skipper N, Thomas D Y, Lau P C
出版信息
EMBO J. 1984 Jan;3(1):107-11. doi: 10.1002/j.1460-2075.1984.tb01769.x.
Abstract
Complementary DNA (cDNA) copies of the M1-1, toxin-coding region of the yeast M1 double-stranded RNA (dsRNA) have been cloned and sequenced. These sequences, in combination with the known terminal sequence of M1-1 dsRNA, identify a translation reading frame for a 316 amino acid protein of 34.7 kd, similar in size to the preprotoxin produced from M1 dsRNA by in vitro translation. Potential glycosylation sites in the preprotoxin peptide are identified. Based on its methionine content the extracellular yeast toxin appears to be contained within the C-terminal region of the precursor.
摘要
已克隆并测序了酵母M1双链RNA（dsRNA）的毒素编码区M1-1的互补DNA（cDNA）拷贝。这些序列与已知的M1-1 dsRNA末端序列相结合，确定了一个34.7 kd、由316个氨基酸组成的蛋白质的翻译阅读框，其大小与通过体外翻译从M1 dsRNA产生的前原毒素相似。确定了前原毒素肽中的潜在糖基化位点。根据其甲硫氨酸含量，细胞外酵母毒素似乎包含在前体的C末端区域内。
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