Lee Blaire, Sosnovtseva Olga, Sørensen Charlotte M, Postnov Dmitry D
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Department of Neuroscience, Aarhus University, Aarhus, Denmark.
Biomed Opt Express. 2022 Mar 21;13(4):2312-2322. doi: 10.1364/BOE.451014. eCollection 2022 Apr 1.
Laser speckle contrast imaging is a robust and versatile blood flow imaging tool in basic and clinical research for its relatively simple construction and ease of customization. One of its key features is the scalability of the imaged field of view. With minimal changes to the system or analysis, laser speckle contrast imaging allows for high-resolution blood flow imaging through cranial windows or low-resolution perfusion visualization of perfusion over large areas, e.g. in human skin. We further utilize this feature and introduce a multi-scale laser speckle contrast imaging system, which we apply to study vasoreactivity in renal microcirculation. We combine high resolution (small field of view) to segment blood flow in individual vessels with low resolution (large field of view) to monitor global blood flow changes across the renal surface. Furthermore, we compare their performance when analyzing blood flow dynamics potentially associated with a single nephron and show that the previously published approaches, based on low-zoom imaging alone, provide inaccurate results in such applications.
激光散斑对比成像因其结构相对简单且易于定制，是基础研究和临床研究中一种强大且通用的血流成像工具。其关键特性之一是成像视野的可扩展性。对系统或分析进行最小程度的更改，激光散斑对比成像就能通过颅骨视窗进行高分辨率血流成像，或对大面积区域（如人体皮肤）进行低分辨率灌注可视化。我们进一步利用这一特性，引入了一种多尺度激光散斑对比成像系统，并将其应用于研究肾微循环中的血管反应性。我们将高分辨率（小视野）用于分割单个血管中的血流，同时将低分辨率（大视野）用于监测整个肾脏表面的全局血流变化。此外，我们在分析可能与单个肾单位相关的血流动力学时比较了它们的性能，并表明之前仅基于低倍率成像的已发表方法在此类应用中会提供不准确的结果。
