使用太赫兹衰减全反射光谱对一种生物活性成分在体外对胶质瘤细胞的影响进行无标记研究。
Label-free study on the effect of a bioactive constituent on glioma cells in vitro using terahertz ATR spectroscopy.
作者信息
Liao Yunsheng, Zhang Mingkun, Tang Mingjie, Chen Ligang, Li Xueqin, Liu Zhongdong, Wang Huabin
机构信息
Research Center of Super-Resolution Optics & Chongqing Engineering Research Center of High-Resolution and Three-Dimensional Dynamic Imaging Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
School of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China.
出版信息
Biomed Opt Express. 2022 Mar 22;13(4):2380-2392. doi: 10.1364/BOE.452952. eCollection 2022 Apr 1.
In this work, we report that the effect of bioactive constituent on living glioma cells can be evaluated using terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy in a label-free, non-invasive, and fast manner. The measured THz absorption coefficient of human glioma cells (U87) in cell culture media increases with ginsenoside Rg3 (G-Rg3) concentration in the range from 0 to 50 µM, which can be interpreted as that G-Rg3 deteriorated the cellular state. This is supported either by the cell growth inhibition rate measured using a conventional cell viability test kit or by the cellular morphological changes observed with fluorescence microscopy. These results verify the effectiveness of using the THz TD-ATR spectroscopy to detect the action of G-Rg3 on glioma cells in vitro. The demonstrated technique thus opens a new route to assessing the efficacy of bioactive constituents on cells or helping screen cell-targeted drugs.
在本研究中，我们报告了可使用太赫兹时域衰减全反射（THz TD - ATR）光谱以无标记、非侵入且快速的方式评估生物活性成分对活胶质瘤细胞的影响。在细胞培养基中，所测得的人胶质瘤细胞（U87）的太赫兹吸收系数随人参皂苷Rg3（G - Rg3）浓度在0至50 μM范围内增加，这可解释为G - Rg3使细胞状态恶化。这一结论得到了使用传统细胞活力检测试剂盒测得的细胞生长抑制率以及通过荧光显微镜观察到的细胞形态变化的支持。这些结果证实了使用太赫兹TD - ATR光谱检测G - Rg3对体外胶质瘤细胞作用的有效性。因此，所展示的技术为评估生物活性成分对细胞的功效或帮助筛选细胞靶向药物开辟了一条新途径。
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