Misrahi M, Atger M, d'Auriol L, Loosfelt H, Meriel C, Fridlansky F, Guiochon-Mantel A, Galibert F, Milgrom E
Biochem Biophys Res Commun. 1987 Mar 13;143(2):740-8. doi: 10.1016/0006-291x(87)91416-1.
A lambda gt10 library containing DNAs complementary to messenger RNAs from human breast cancer T47-D cells was constructed and screened with a cDNA probe encoding the rabbit progesterone receptor. Four overlapping clones have been sequenced. The open reading frame corresponds to a protein of 933 amino acids with a molecular weight of 98,868 Da. The cysteine rich basic region supposed to be involved in DNA binding is completely homologous in the human and rabbit receptors, whereas the C-terminal end, where hormone binding is thought to take place, differs by a single amino acid change. The human progesterone receptor is characterized, as is the rabbit receptor, by the very high proline content of its N-terminal region. When mRNAs from either human breast cancer cell lines T47-D and MCF-7 or from normal human uterus tissue were blotted and probed with the cloned cDNA, four main bands were observed (5100, 4300, 3700, and 2900 nucleotides).
构建了一个λgt10文库，其包含与人乳腺癌T47 - D细胞信使RNA互补的DNA，并使用编码兔孕酮受体的cDNA探针进行筛选。已对四个重叠克隆进行了测序。开放阅读框对应于一个由933个氨基酸组成的蛋白质，分子量为98,868道尔顿。推测参与DNA结合的富含半胱氨酸的碱性区域在人和兔受体中完全同源，而被认为发生激素结合的C末端仅相差一个氨基酸变化。人孕酮受体与兔受体一样，其N末端区域脯氨酸含量非常高。当用人乳腺癌细胞系T47 - D和MCF - 7或正常人子宫组织的mRNA进行印迹并用克隆的cDNA进行探测时，观察到四条主要条带（5100、4300、3700和2900个核苷酸）。
