Loosfelt H, Atger M, Misrahi M, Guiochon-Mantel A, Meriel C, Logeat F, Benarous R, Milgrom E
Proc Natl Acad Sci U S A. 1986 Dec;83(23):9045-9. doi: 10.1073/pnas.83.23.9045.
Two lambda gt11 clones containing fragments of cDNA encoding the rabbit progesterone receptor were isolated with the aid of monoclonal and monospecific polyclonal antireceptor antibodies. RNA gel blot analysis showed that the corresponding mRNA was approximately equal to 5900 nucleotides in size and present in the uterus, where its concentration was increased by estrogen treatment, and in the vagina. This mRNA was not detected in liver, in spleen, in intestine, and in kidney where the receptor protein is known to be absent or present in very small concentration. Cross-hybridizing clones were isolated from a lambda 10 library. The DNA was sequenced, and the primary structure of the progesterone receptor was deduced. It consists of 930 amino acids and contains a basic, cysteine-rich region (residues 568-645) with extensive homology to the glucocorticoid and estrogen receptors and the v-erbA oncogene protein. This region is followed by a C-terminal domain that is similar in size to the corresponding domains of the other steroid receptors and v-erbA and shows striking amino acid homology with the glucocorticoid receptor and significant homology with the estrogen receptor. In contrast, the region extending from the cysteine-rich segment toward the N terminus differed in size and amino acid sequence from that of the other receptors and v-erbA. This region had a high proline content in the progesterone receptor.
借助单克隆和单特异性多克隆抗受体抗体，分离出了两个含有编码兔孕激素受体cDNA片段的λgt11克隆。RNA凝胶印迹分析表明，相应的mRNA大小约为5900个核苷酸，存在于子宫中，其浓度经雌激素处理后会增加，在阴道中也有表达。在已知不存在受体蛋白或受体蛋白浓度极低的肝脏、脾脏、肠道和肾脏中未检测到这种mRNA。从λ10文库中分离出了交叉杂交克隆。对DNA进行了测序，并推导了孕激素受体的一级结构。它由930个氨基酸组成，包含一个碱性的、富含半胱氨酸的区域（第568 - 645位氨基酸残基），与糖皮质激素受体、雌激素受体以及v-erbA癌基因蛋白具有广泛的同源性。该区域之后是一个C末端结构域，其大小与其他类固醇受体和v-erbA的相应结构域相似，与糖皮质激素受体具有显著的氨基酸同源性，与雌激素受体具有明显的同源性。相比之下，从富含半胱氨酸的片段向N末端延伸的区域，其大小和氨基酸序列与其他受体和v-erbA不同。该区域在孕激素受体中脯氨酸含量较高。
