Zhao Yanhui, Wang Miao, Sun Lin, Jiang Xue, Zhao Min, Zhao Chunjie
School of Pharmacy, Shenyang Pharmaceutical University Wenhua Road 103 Shenhe District Shenyang 110016 Liaoning Province China
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University Wenhua Road 103 Shenyang Liaoning Province China.
RSC Adv. 2020 Jul 10;10(44):26109-26119. doi: 10.1039/d0ra02264k. eCollection 2020 Jul 9.
Sanhua decoction, a famous Chinese herbal formula has been widely used for the treatment of stroke. In our study, a rapid, swift and straightforward analytical method with the help of UHPLC-FT-ICR-MS/MS was successfully developed for the first time to separate and identify the chemical constituents of Sanhua decoction. Chromatography was performed on a Universal XB C column (150 mm × 2.1 mm, 1.8 μm) using a mobile phase containing 0.1% formic acid-water (A) and acetonitrile (B). A total of 137 compounds in Sanhua decoction were identified or tentatively characterized. The findings revealed the fact that Sanhua decoction mainly contains flavonoids (in and L.), anthraquinones (in L.), coumarins (in ), phenylpropanoid glycosides, alkaloids and lignans (in ), which made up the key ingredients existing in Sanhua decoction. This study is hoped to be meaningful for the characterization of components in other traditional Chinese medicines, and lay the foundation for research on the pharmacology of Sanhua decoction.
三花汤是一种著名的中药方剂，已被广泛用于治疗中风。在我们的研究中，首次成功开发了一种借助超高效液相色谱-傅里叶变换离子回旋共振质谱联用仪（UHPLC-FT-ICR-MS/MS）的快速、便捷且直接的分析方法，用于分离和鉴定三花汤的化学成分。色谱分析在通用XB C柱（150 mm×2.1 mm，1.8μm）上进行，流动相为含0.1%甲酸的水（A）和乙腈（B）。三花汤中总共鉴定出或初步表征了137种化合物。研究结果表明，三花汤主要含有黄酮类化合物（在[具体植物名称1]和[具体植物名称2]中）、蒽醌类化合物（在[具体植物名称3]中）、香豆素类化合物（在[具体植物名称4]中）、苯丙素苷、生物碱和木脂素类化合物（在[具体植物名称5]中），这些构成了三花汤中的关键成分。本研究有望对其他中药成分的表征具有意义，并为三花汤的药理学研究奠定基础。
