Bi Junying, Zhang Haiyan, Lu Jing, Lei Weifu
Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
Gynaecology Ward‑1, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China.
Mol Med Rep. 2016 Dec;14(6):5408-5414. doi: 10.3892/mmr.2016.5919. Epub 2016 Nov 1.
A recent study reported that nobiletin is an active ingredient in Fructus Aurantii immaturus and Pericarpium Citri Reticulatae, which may be capable of preventing ischemic stroke. Therefore, the present study aimed to determine the neuroprotective effects of nobiletin, and to evaluate whether it could ameliorate isoflurane‑induced cognitive impairment via antioxidant, anti‑inflammatory and anti‑apoptotic effects in aging rats. Male Sprague‑Dawley rats (age, 18 months) were used to analyze the neuroprotective effects of nobiletin. Morris water maze test was used to determine cognitive competence. Enzyme‑linked immunosorbent assay and western blot analysis were also used to quantify nuclear factor‑κB, tumor necrosis factor (TNF)‑α, IL‑1β, IL‑6, glutathione, (GSH), GSH‑peroxidase, superoxide dismutase and malondialdehyde concentration and relevant protein expression levels Cognitive competence was increased in isoflurane-treated rats following treatment with nobiletin. In addition, as expected, nobiletin exerted antioxidant, anti-inflammatory and anti‑apoptotic effects on isoflurane‑induced cognitive impairment in aging rats. Treatment with nobiletin induced the activation of phosphorylated (p)‑Akt, p‑cAMP response element binding protein (CREB) and brain‑derived neurotrophic factor (BDNF) protein expression and reduced the levels of B‑cell lymphoma 2‑associated X protein (Bax) in isoflurane‑induced rats. In conclusion, the present study demonstrated that nobiletin may ameliorate isoflurane-induced cognitive impairment through antioxidant, anti‑inflammatory and anti‑apoptotic effects via modulation of Akt, Bax, p‑CREB and BDNF in aging rats. These findings provide support for the molecular mechanisms underlying the effects of nobiletin treatment on isoflurane-induced damage.
最近的一项研究报告称，诺米林是枳实和陈皮中的一种活性成分，可能具有预防缺血性中风的能力。因此，本研究旨在确定诺米林的神经保护作用，并评估其是否能通过抗氧化、抗炎和抗凋亡作用改善老龄大鼠异氟烷诱导的认知障碍。采用雄性Sprague-Dawley大鼠（18月龄）分析诺米林的神经保护作用。采用Morris水迷宫试验测定认知能力。还采用酶联免疫吸附测定和蛋白质印迹分析来量化核因子-κB、肿瘤坏死因子（TNF）-α、白细胞介素-1β、白细胞介素-6、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶、超氧化物歧化酶和丙二醛浓度以及相关蛋白表达水平。诺米林治疗后，异氟烷处理的大鼠认知能力增强。此外，正如预期的那样，诺米林对老龄大鼠异氟烷诱导的认知障碍具有抗氧化、抗炎和抗凋亡作用。诺米林治疗可诱导异氟烷诱导的大鼠磷酸化（p）-Akt、p-环磷酸腺苷反应元件结合蛋白（CREB）的激活和脑源性神经营养因子（BDNF）蛋白表达，并降低B细胞淋巴瘤2相关X蛋白（Bax）水平。总之，本研究表明，诺米林可能通过调节老龄大鼠的Akt、Bax、p-CREB和BDNF，通过抗氧化、抗炎和抗凋亡作用改善异氟烷诱导认知障碍。这些发现为诺米林治疗异氟烷诱导损伤的分子机制提供了支持。
