Zhang Jun, Peng Ching-An
Department of Biological Engineering, University of Idaho Engineering Physics Building 421, 875 Perimeter Drive Moscow ID 83844-0904 USA
RSC Adv. 2019 Mar 1;9(13):7156-7164. doi: 10.1039/c8ra09768b.
Carbon nanotubes (CNTs) have been extensively studied for photothermal ablation of malignant cells due to their ability to absorb near-infrared (NIR) laser light and convert it to thermal energy for the lysis of tumor cells. Functionalizing CNTs with tumor-targeting moieties can facilitate the delivery to tumor sites. Instead of using targeting moieties, mesenchymal stem cells (MSCs) have been considered as vehicles to deliver therapeutic agents to cancer cells. In this study, the effects of attaching CNTs to MSCs on cell migration in response to a chemotactic gradient were investigated. Multiwalled carbon nanotubes (MWCNTs) were functionalized with streptavidin-fluorescein isothiocyanate (SA-FITC). The surface of human MSCs was biotinylated by culturing MSCs with biotin-lipid containing medium. CNTs were then attached on the outer cell membrane of biotinylated MSCs through SA-biotin binding. Fluorescence microscopy confirmed CNTs were located on the surface of MSCs. Various amounts of CNTs anchored on the membrane of MSCs were used to examine the effects of CNTs on MSC proliferation and migration. Our transwell migration assay showed that 4.26 ng CNT per cell is the threshold value that would not affect the migration speed of CNT-tagged MSCs toward the established gradient of chemoattractant SDF-1α.
由于碳纳米管（CNTs）能够吸收近红外（NIR）激光并将其转化为热能以裂解肿瘤细胞，因此已被广泛研究用于恶性细胞的光热消融。用肿瘤靶向部分对碳纳米管进行功能化可以促进其向肿瘤部位的递送。除了使用靶向部分外，间充质干细胞（MSCs）也被认为是将治疗剂递送至癌细胞的载体。在本研究中，研究了将碳纳米管附着于间充质干细胞对趋化梯度响应下细胞迁移的影响。用链霉亲和素 - 异硫氰酸荧光素（SA - FITC）对多壁碳纳米管（MWCNTs）进行功能化。通过用含生物素 - 脂质的培养基培养间充质干细胞，使其表面生物素化。然后通过SA - 生物素结合将碳纳米管附着在生物素化间充质干细胞的外细胞膜上。荧光显微镜证实碳纳米管位于间充质干细胞表面。使用锚定在间充质干细胞膜上的各种量的碳纳米管来检查碳纳米管对间充质干细胞增殖和迁移的影响。我们的Transwell迁移试验表明，每个细胞4.26 ng碳纳米管是不会影响碳纳米管标记的间充质干细胞向已建立的趋化因子SDF - 1α梯度迁移速度的阈值。
