Lara-Martínez Luis A, Massó Felipe, Palacios González Eduardo, García-Peláez Isabel, Contreras-Ramos Alejandra, Valverde Mahara, Rojas Emilio, Cervantes-Sodi Felipe, Hernández-Gutiérrez Salomón
Department of Molecular Biology, School of Medicine, Universidad Panamericana, Mexico City, Mexico.
Department of Physiology, National Institute of Cardiology Ignacio Chavez, Mexico City, Mexico.
Int J Nanomedicine. 2017 Oct 19;12:7695-7707. doi: 10.2147/IJN.S144777. eCollection 2017.
Carbon nanotubes (CNTs) have been a focus of attention due to their possible applications in medicine, by serving as scaffolds for cell growth and proliferation and improving mesenchymal cell transplantation and engraftment. The emphasis on the benefits of CNTs has been offset by the ample debate on the safety of nanotechnologies. In this study, we determine whether functionalized multiwalled CNTs (fMWCNTs) and functionalized oxygen-doped multiwalled CNTs (fCOxs) have toxic effects on rat mesenchymal stem cells (MSCs) in vitro by analyzing morphology and cell proliferation and, using in vivo models, whether they are able to transform MSCs in cancer cells or induce embryotoxicity. Our results demonstrate that there are statistically significant differences in cell proliferation and the cell cycle of MSCs in culture. We identified dramatic changes in cells that were treated with fMWCNTs. Our evaluation of the transformation to cancer cells and cytotoxicity process showed little effect. However, we found a severe embryotoxicity in chicken embryos that were treated with fMWCNTs, while fCOxs seem to exert cardioembryotoxicity and a discrete teratogenicity. Furthermore, it seems that the time of contact plays an important role during cell transformation and embryotoxicity. A single contact with fMWCNTs is not sufficient to transform cells in a short time; an exposure of fMWCNTs for 2 weeks led to cell transformation risk and cardioembryotoxicity effects.
碳纳米管（CNTs）因其在医学领域的潜在应用而备受关注，它可作为细胞生长和增殖的支架，并改善间充质细胞移植和植入。然而，关于纳米技术安全性的大量争论抵消了对碳纳米管益处的强调。在本研究中，我们通过分析形态和细胞增殖来确定功能化多壁碳纳米管（fMWCNTs）和功能化氧掺杂多壁碳纳米管（fCOxs）在体外对大鼠间充质干细胞（MSCs）是否具有毒性作用，并利用体内模型确定它们是否能够将间充质干细胞转化为癌细胞或诱导胚胎毒性。我们的结果表明，培养的间充质干细胞在细胞增殖和细胞周期方面存在统计学上的显著差异。我们发现用fMWCNTs处理的细胞有显著变化。我们对癌细胞转化和细胞毒性过程的评估显示影响较小。然而，我们发现用fMWCNTs处理的鸡胚存在严重的胚胎毒性，而fCOxs似乎会产生心脏胚胎毒性和轻微的致畸性。此外，接触时间似乎在细胞转化和胚胎毒性过程中起着重要作用。单次接触fMWCNTs不足以在短时间内转化细胞；暴露于fMWCNTs两周会导致细胞转化风险和心脏胚胎毒性效应。
